brazilian wandering spider neurotoxin

Brazilian wandering spiders: Bites & other facts

The spider's name means "murderess" in Greek, which is appropriate for the deadly arachnid.

Classification/taxonomy

Size & characteristics, bites and venom, additional resources.

The Brazilian wandering spider, also called armed spiders or banana spiders, belongs to the genus Phoneutria , which means "murderess" in Greek. And it's no wonder why — it's one of the most venomous spiders on Earth . Its bite, which delivers neurotoxic venom, can be deadly to humans, especially children, although antivenom makes death unlikely.

Guinness World Records has previously named the Brazilian wandering spider the world's most venomous spider multiple times (though the current record-holder is the Sydney funnel-web spider, Atrax robustus , according to Guinness ). But, as the late Jo-Anne Sewlal, who was an arachnologist at the University of the West Indies in Trinidad and Tobago, told Live Science, "classifying an animal as deadly is controversial," as the amount of damage depends on the amount of venom injected. 

Jo-Anne Sewlal was a noted arachnologist from Trinidad and Tobago. While completing her PhD, she received the National Institute of Higher Education, Research, Science and Technology (NIHERST) 2012 Award for Excellence in Science and Technology for Junior Scientist. In 2013, She received a doctorate in zoology from the University of the West Indies. She discovered several species of spiders in her home country, surveyed the arachnids across several countries the Caribbean and appeared as an expert on the topic on The Science Channel. She died of an allergic reaction in January 2020.

There are nine species of Brazilian wandering spider, all of which are nocturnal and can be found in Brazil. Some species also can be found throughout Central and South America, from Costa Rica to Argentina, according to a 2008 article in the journal American Entomologist . Study author Richard S. Vetter, a research associate in the department of entomology at the University of California, Agriculture and Natural Resources, wrote that specimens of these powerful arachnids have been mistakenly exported to North America and Europe in banana shipments. However, Vetter noted, in many cases of cargo infestation, the spider in question is a harmless banana spider (genus Cupiennius ) that is misidentified as a Phoneutria . The two types of spiders look similar.

The taxonomy of Brazilian wandering spiders, according to the Integrated Taxonomic Information System (ITIS) , is:

Kingdom : Animalia Subkingdom : Bilateria Infrakingdom : Protostomia Superphylum : Ecdysozoa Phylum : Arthropoda Subphylum : Chelicerata Class : Arachnida Order : Araneae Family : Ctenidae Genus : Phoneutria  

• Phoneutria bahiensis
• Phoneutria boliviensis
• Phoneutria eickstedtae
• Phoneutria fera
• Phoneutria keyserlingi
• Phoneutria nigriventer
• Phoneutria pertyi
• Phoneutria reidyi
• Phoneutria depilata , according to a 2021 study published in the journal ZooKeys , which found that Phoneutria boliviensis actually included two separate species from different habitats. 

Brazilian wandering spiders are large, with bodies reaching up to 2 inches (5 centimeters) and a leg span of up to 7 inches (18 cm), according to the Natural History Museum in Karlsruhe, Germany. The species vary in color, though all are hairy and mostly brown and gray, although some species have lightly colored spots on their abdomen. Many species have bands of black and yellow or white on the underside of the two front legs, according to the University of Florida . 

These arachnids "are called wandering spiders because they do not build webs but wander on the forest floor at night, actively hunting prey," Sewlal told Live Science in an interview conducted in 2014, before her death. They kill by both ambush and direct attack.

They spend most of their day hiding under logs or in crevices, and come out to hunt at night. They eat insects, other spiders and sometimes, small amphibians, reptiles and mice. 

Research into one species of Brazilian wandering spider, Phoneutria boliviensis , revealed that these spiders eat a mix of arthropods and reptiles. DNA metabarcoding, a technique that examines the DNA and RNA in a sample, of the guts of 57 spiders identified 96 prey species, including flies, beetles, butterflies, moths, grasshoppers, locusts and crickets, according to research from the University of Tolima and the University of Ibagué in Colombia . Some of the female spiders also ate lizards and snakes.

While their bites are powerful and painful, "their bites are a means of self-defense and only done if they are provoked intentionally or by accident," Sewlal said.

In the Brazilian wandering spider, just as in most spider species, the female is larger than the male. Males approach females cautiously when attempting to mate, according to the biology department at the University of Wisconsin-La Crosse . Males perform a dance to get females' attention, and males often fight each other over the female. The female can be picky, and she often turns down many males before choosing a mating partner. Once she does pick one, the male needs to watch out; females often attack the males once copulation is finished.

The female then can store the sperm in a separate chamber from the eggs until she is ready to fertilize them. She will lay up to 1,000 eggs at a time, which are kept safe in a spun-silk egg sac.

Brazilian wandering spiders typically live for one or two years.

Brazilian wandering spiders' venom is a complex cocktail of toxins, proteins and peptides, according to the Natural History Museum in Karlsruhe, Germany. The venom affects ion channels and chemical receptors in victims' neuromuscular systems.

After a human is bitten by one of these spiders, he or she may experience initial symptoms such as severe burning pain at the site of the bite, sweating and goosebumps, Sewlal said. Within 30 minutes, symptoms become systemic and include high or low blood pressure , fast or a slow heart rate , nausea, abdominal cramping, hypothermia, vertigo, blurred vision, convulsions and excessive sweating associated with shock. People who are bitten by a Brazilian wandering spider should seek medical attention immediately.

Their  venom is perhaps most famous for triggering painful and long-lasting erections . For that reason, in a 2023 study, scientists reported that they were testing the venom in humans as a potential treatment for erectile dysfunction in those for whom Viagra didn't work.

However, these bites are rare, and envenomations, or exposure to these toxins from a spider bite, are usually mild, Vetter said. For instance, a 2000 study in the journal Revista do Instituto de Medicina Tropical de São Paulo found that only 2.3% of people with bites who came to a Brazilian hospital over a 13-year period were treated with antivenom. (The other bites did not contain enough venom to require it.) Most of the bites were from the species P. nigriventer and P. keyserlingi in eastern coastal Brazil. About 4,000 bites reportedly happen each year in Brazil, but only 0.5% of those cases are severe, according to a 2018 study in the journal Clinical Toxinology in Australia, Europe, and Americas . Meanwhile, 15 deaths have been attributed to Phoneutria in Brazil since 1903, the 2018 study reported. 

"It is unlikely that the spider would inject all of its venom into you, as this venom is not only needed as a means of defense but to immobilize prey," Sewlal said. "So if it did inject all of its venom, it [would] have to wait until its body manufactured more before it could hunt." That would also leave the spider vulnerable to being attacked by predators.

Furthermore, Sewlal pointed out that venom production requires a lot of a spider's resources and time. "So if the spider were to attack frequently and use up all of its venom, it [would] be safe to assume that it has a ready food supply to replace the energy and resources used. This situation does not exist in the wild."

• Learn more about Brazilian wandering spiders from the University of Wisconsin-La Crosse .
• Read about several species of Brazilian wandering spiders, including several images of the arachnids at the University of Florida .
• Find a spider in your bananas? It may or may not be a deadly species, according to the University of California, Riverside .

This article was originally published on Nov. 20, 2014. 

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Jessie Szalay is a contributing writer to FSR Magazine. Prior to writing for Live Science, she was an editor at Living Social. She holds an MFA in nonfiction writing from George Mason University and a bachelor's degree in sociology from Kenyon College. 

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Brazilian Wandering Spider

The Brazilian Wandering Spider (Phoneutria fera) is an aggressive and highly venomous spider . It was first discovered in Brazil hence its name. However, this genus is known to exist elsewhere in South and Central America .

The Brazilian Wandering spider is a member of the Ctenidae family of wandering spiders.

The Brazilian Wandering spider appeared in the Guinness Book of World Records 2007 for being the most venomous animal .

In this particular genus, there are five known similar species whose members are also highly venomous. They include some of the relatively few species of spiders that present a threat to human beings.

Brazilian Wandering Spider Characteristics

The Brazilian wandering spider can grow to have a leg span of up to 4 – 5 inches. They are large hairy spindly-looking spiders who have eight eyes, two of which are large. Brazilian wandering spiders are fast-moving spiders, their legs are strong and spiny and they have distinctive red jaws which they display when angered.

The Brazilian wandering spider is not a Tarantula . Brazilian wandering spiders are not even in the same family group. Tarantulas are harmless to humans and are mostly ambush killers who wait for prey to come to them. Brazilian wandering spiders are active hunters. Brazilian wandering spiders and Tarantulas do have one thing in common, however, they do not eat bananas.

Brazilian Wandering Spider Habitat and Spider Webs

The Brazilian Wandering spider is so-called because it wanders the jungle floor, rather than residing in a lair or maintaining a web. This is another reason it is considered so dangerous. In densely populated areas, the Brazilian Wandering spider will usually search for cover and dark places to hide during daytime, leading it to hide within houses, clothes, cars, boots, boxes and log piles. This usually causes accidents when people disturb them.

The Brazilian Wandering spider is also called the ‘banana spider’ as it is occasionally found within shipments of bananas. As a result, any large spider appearing in a bunch of bananas should be treated with due care.

Brazilian Wandering Spider Diet

Adult Brazilian Wandering spiders eat crickets, other large insects, small lizards and mice. Spiderlings of this species eat flightless fruit flies and pinhead crickets.

Brazilian Wandering Spider Reproduction

All spiders produce silk, a thin, strong protein strand extruded by the spider from spinnerets most commonly found on the end of the abdomen. Many species use it to trap insects in webs, although there are many species that hunt freely such as the Brazilian Wandering spider. Silk can be used to aid in climbing, form smooth walls for burrows, build egg sacs, wrap prey and temporarily hold sperm, among other applications.

Brazilian Wandering spiders reproduce by means of eggs, which are packed into silk bundles called egg sacs. The male spider must (in most cases) make a timely departure after mating to escape before the females normal predatory instincts return.

Mature male spiders have swollen bulbs on the end of their palps for this purpose and this is a useful way to identify whether the spider is male or female. Once the sperm is inside the female spider, she stores it in a chamber and only uses it during the egg-laying process, when the eggs come into contact with the male sperm for the first time and are fertilized. The Brazilian Wandering spiders life cycle is 1 – 2 years.

Brazilian Wandering Spider Venom

Bites from the Brazilian Wandering spider may result in only a couple of painful pinpricks to full-blown envenomed. In either case, people bitten by this spider or any Ctenid should seek immediate emergency treatment as the venom is possibly life threatening.

The Phoneutria fera and Phoneutria nigriventer (two species of wandering spider) are the two most commonly implicated as the most vicious and deadly of the Phoneutria spiders.

The Phoneutria not only has a potent neurotoxin, but is reported to have one of the most excruciatingly painful envenoms of all spiders due to its high concentration of serotonin. They have the most active venom of any living spiders.

One of their members, the Brazilian Huntsman, is thought to be the most venomous spider in the world. Brazilian wandering spiders are certainly dangerous and bite more people than any other spiders.

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• J Venom Anim Toxins Incl Trop Dis

Gene sequence analysis of toxins from the spider Phoneutria nigriventer revealed an intronless feature

Ana luiza bittencourt paiva.

1 Diretoria de Pesquisa e Desenvolvimento, Fundação Ezequiel Dias (FUNED), Belo Horizonte, MG, Brazil.

Alessandra Matavel

Bruno césar souza silva.

2 Universidade Federal de Minas Gerais (UFMG), Programa Interunidades de Pós-graduação em Bioinformática (ICB), Belo Horizonte, MG, Brazil.

Clara Guerra-Duarte

Marcelo ribeiro vasconcelos diniz.

Authors' contributions: ALBP performed experiments and analysis; participated in the design and interpretation of the data; wrote the paper and participated in its revisions. AM wrote the paper and participated in its revisions. BCSS oerformed the bioinformatics analysis. CGD wrote the paper and participated in its revisions. MRVD conceived this research and designed the experiments. All authors read and approved the final manuscript.

Background:

Phoneutria nigriventer spider venom contains several cysteine-rich peptide toxins that act on different ion channels. Despite extensive studies on its venom and description of cDNA sequences of several of its toxin precursors, the gene structure of these toxins remains unknown.

Genomic regions encoding the precursors of three previously characterized P. nigriventer toxins - PnTx1, PnTx2-5 and PnTx4(5-5) - were amplified by PCR using specific primers. PCR fragments were cloned and sequenced. Obtained sequences were compared with their corresponding cDNA sequences.

The size of PCR fragments obtained and sequences corresponding to genomic regions encoding for the toxin precursors matched their cDNA sequences.

Conclusions:

Despite a few nucleotide substitutions in the genomic regions encoding for the toxin precursors when compared with cDNA sequences, the results of the present work indicate that P. nigriventer toxins do not contain introns in their genes sequences.

Phoneutria nigriventer is one of the largest existing spiders from the suborder Araneomorphae (RTA clade; family Ctenidae) and one of the few in the world that can cause harm to humans [ 1 , 2 ]. They are wandering, solitary and aggressive spiders, relying on strength and venom toxicity for defense and prey capture rather than using silk webs. P. nigriventer venom contains several neurotoxic peptides that act on ion channels and chemical receptors of vertebrates and invertebrates [ 3 ].

Although this venom has been studied for over 40 years and cDNA sequences of several toxin precursors have been obtained [ 4 - 10 ], up to this moment there is no investigation on the genome or even gene structure of any of its toxins.

The earliest spiders dates back to about 300 million years ago. Due to this long existence, together with the high species diversity in this group, spiders have been studied as an interesting evolution model. As an important trait for their evolutionary success, the study of venom and toxin evolution in the molecular level may contribute in elucidating the complex history of spiders [ 11 ]. Morphological and behavioral data have been traditionally used to infer phylogeny, but genomic and transcriptomic molecular data are recently challenging previous assumptions on the tree of life of spiders [ 12 , 13 ]. The genetic architecture of their toxin genes has shown to be variable in different spider major groups - such as Araneomorphs [ 14 ] and Mygalomorphs [ 15 - 18 ] -, and genes encoding for venom toxins have been described both with and without introns. Therefore, the description of Phoneutria toxins gene structure, as a member of the RTA clade (the most diverse group within spiders), is a relevant contribution to the field.

Several novel components of high molecular mass have been described in P. nigriventer venom, but cysteine-rich peptide toxins are by far its most abundant component [ 4 ]. PnTx1 (μ-ctenitoxin-Pn1a), PnTx2-5 (δ-ctenitoxin-Pn2c) and PnTx4(5-5) (ɣ-ctenitoxin-Pn1a) are among the best characterized and most abundant cysteine-rich peptide toxins in P. nigriventer venom. PnTx1 was demonstrated to inhibit sodium channel currents [ 19 , 20 ] and has shown great neurotoxicity, inducing tail elevation, excitation, salivation, spastic paralysis and lethality in mice [ 21 ]. PnTx2-5 acts on sodium channels as well, being able to inhibit sodium channel inactivation [ 22 ]. It is also one of the P. nigriventer ’s most toxic venom components to mice [ 23 ] and can induce penile erection, hypersalivation and death by respiratory distress or pulmonary edema [ 24 ]. Unlike these two first toxins, PnTx4(5-5) is toxic only to insects [ 25 ], showing a remarkable effect on insect sodium channels inactivation [ 26 ]. This toxin also seems to hold a biotechnological potential use as a neuroprotective [ 27 ] and analgesic drug lead [ 28 ].

Considering the relevance of P. nigriventer toxins and due to their biotechnological, medical and evolutionary importance, we have investigated the gene structure of these three sodium channel modulators toxins from P. nigriventer venom, searching for the presence or absence of introns.

Venom glands and genomic DNA (gDNA) were obtained from P. nigriventer adult spiders maintained at Ezequiel Dias Foundation in Belo Horizonte, Brazil (Sisgen #A26E945).

Isolation of RNA and cDNA synthesis

Total RNA was extracted from pooled venom glands of five adult specimens using TRIzol reagent (Invitrogen, USA) according to the manufacturer’s protocol. Subsequently, total RNA was used to synthesize cDNA first strand using Super Script First-Strand Synthesis System for RT-PCR kit (Invitrogen, USA), following the manufacturer’s protocol.

Isolation of genomic DNA

Genomic DNA (gDNA) was extracted from the leg muscular tissue of an adult P. nigriventer specimen, as described by Fan and Gulley [ 29 ]. After spider euthanasia, two legs were isolated from the base of the cephalothorax and ground with a chilled, sterile mortar and pestle in liquid nitrogen. After pulverization, the tissue was added to 450 µL of extraction buffer (0.01 M NaCl, 20 mM Tris- HCl pH 8, 1 mM EDTA, 1% SDS and 300 µg/mL proteinase K) and incubated at 55°C for 3h. One volume of phenol:chloroform:isoamyl alcohol (25:24:1) was added and the aqueous phase was collected after centrifugation. DNA was recovered by adding 50 µL of 3 M NaOAc pH 5.2, followed by ethanol precipitation and re-suspended in 100 µL of TE buffer (Tris 10mM, EDTA 1mM).

Amplification and sequencing of toxin genes

Sequences of gDNA and cDNA encoding for toxins were amplified by PCR using specific primers ( Table 1 ). Primers sequences for PnTx1 and PnTx2-5 precursors were designed using information from cDNA sequences deposited in GenBank database (accession numbers {"type":"entrez-nucleotide","attrs":{"text":"X73155.1","term_id":"396255","term_text":"X73155.1"}} X73155.1 and {"type":"entrez-nucleotide","attrs":{"text":"AF014463.1","term_id":"3319220","term_text":"AF014463.1"}} AF014463.1 , respectively). Primers for PnTx4(5-5) precursor and mature sequences were designed using a cDNA sequence obtained from a P. nigriventer venom glands transcriptome study [ 4 ].

PCR reactions contained 100 ng of gDNA or cDNA, 1x PCR buffer, 1 μM dNTPs, 1 μM of each primer and 1 U Platinum Taq DNA polymerase High Fidelity (Invitrogen, USA). Cycling conditions were: 5 min at 94ºC followed by 35 cycles at 94°C for 45 s, 50°C for 45 s, 72°C for 1 min, and a final cycle of 72ºC for 7 min. PCR products were subjected to electrophoresis on 2% agarose gel. gDNA fragments extracted from the agarose gel were purified using Illustra GFX PCR DNA and Gel Band Purification kit (GE Healthcare, USA) and cloned into a pGEM-T Easy vector (Promega, USA). Positive clones were sequenced in an ABI PRISMTM 3700 DNA Automatic Sequencer using the standard M13 reverse primer and Big Dye Terminator v3.1 Cycle sequencing kit (Applied Biosystems, USA).

Analysis of intron presence in cysteine-rich peptide neurotoxins in available spider genomes

Sequenced spider genomes available in NCBI Genome public database (accession number for each species are as follows: Loxosceles reclusa - JJRW01; Stegodyphus mimosarum - AZAQ01; Araneous ventricosus - BGPR01; Dysdera sylvatica - QLNU01; Trichonephila clavipes - MWRG01; Acanthoscurria geniculate - AZMS01; Pardosa pseudoannulata - SBLA01) were used to search for cysteine-rich peptide toxins sequences and evaluate the presence of introns within their genes.

Since it was not possible to find a specific cysteine-rich peptide toxin sequence published for each of these spiders species, multi FASTA archives were built with known toxin sequences from other spider species, annotated as inhibitor cysteine knot (ICK), knottin or cysteine-rich peptide toxin retrieved from ArachnoServer [ 30 ], Knottin database [ 31 ] and GenBank [ 32 ] to be used as queries to interrogate the genomes. Genomes were used individually as databases to run BLASTn and tBLASTn [ 33 ], with default parameters. Results were manually inspected, considering hits with e-value < 1e-05, searching for breaks in the obtained alignment, which would indicate the discontinuity of the sequence, pointing to the presence of introns.

Using information from cDNA sequences, we designed specific primers in order to amplify genomic regions encoding for the precursors of three well characterized toxins from the spider P. nigriventer , namely PnTx1, PnTx2-5, and PnTx4(5-5). The forward primers are complementary to the beginning of the sequence region encoding for the toxins signal peptides. Reverse primers were designed to anneal to the 3’ UTR regions.

The results showed that the size of PCR fragments corresponding to amplified gDNA encoding for precursor sequences of PnTx1 and PnTx2-5 matched the amplified cDNA fragments, which were expected to be 396 bp and 271 bp, respectively ( Figure 1 ). This indicates that these two toxins do not contain introns in their precursor gene sequences.

Despite several attempts, we could not obtain any amplification for PnTx4(5-5) using the forward primers annealing to the sequence region encoding for the toxin signal peptide with gDNA as a template. This lack of amplification may be due to the existence of extensive nucleotide variation in DNA sequences encoding for toxins in spiders [ 34 ]. Indeed, it has been demonstrated that PnTx4(5-5) is one of the most expressed toxins in P. nigriventer venom glands, presenting several isoforms [ 4 ]. Thus, we have designed a new forward primer to amplify only the mature sequence of PnTx4(5-5). With this new approach, amplification was observed ( Fig. 1 ) and as for the other tested toxins, gDNA and cDNA amplified fragments presented the same size (181 bp). This indicates that at least the gDNA region encoding for PnTx4(5-5) mature sequence is also intronless.

In order to confirm if the amplified gDNA sequences of PnTx1 and PnTx2-5 matched the cDNA sequences deposited in the GenBank database (accession numbers {"type":"entrez-nucleotide","attrs":{"text":"X73155.1","term_id":"396255","term_text":"X73155.1"}} X73155.1 and {"type":"entrez-nucleotide","attrs":{"text":"AF014463.1","term_id":"3319220","term_text":"AF014463.1"}} AF014463.1 , respectively), the PCR products from gDNA were cloned and sequenced. Each sequence obtained for PnTx1 and PnTx2-5 presented one non-synonymous nucleotide substitution in the propeptide region, leading to one amino acid alteration when compared with the corresponding cDNA sequence deposited in GenBank ( Figure 2 ). Furthermore, PnTx1 gDNA sequence also presented two synonymous substitutions in the toxin mature sequence ( Fig. 2A ) and PnTx2-5 presented one synonymous substitution in the signal peptide sequence ( Fig. 2B ). The gDNA sequences obtained for PnTx1 and PnTx2-5 were deposited in GenBank database under the accession numbers {"type":"entrez-nucleotide","attrs":{"text":"MN851275","term_id":"1843476731","term_text":"MN851275"}} MN851275 and {"type":"entrez-nucleotide","attrs":{"text":"MN851276","term_id":"1843476733","term_text":"MN851276"}} MN851276 , respectively.

Our results were compared with other published data concerning the presence of introns in spider cysteine-rich peptide toxins ( Figure 3 ). Available spider genome sequences were also used for an in-silico analysis, using a multiFASTA archive containing several cysteine-rich peptide toxin sequences. We did not find evidence of introns in the retrieved alignments with the genomic sequences of A. geniculata (Mygalomorphae ) , since all alignments were contiguous with the queries. Although most toxin sequences found in Araneomaphae genomes seem to be contiguous, at least three sequences in A. ventricosus, two sequences in P. pseudoannulata, two sequences in S. mimosarum and two sequences in T. clavipes showed evidence of possible introns, but a more in-depth analysis is required to confirm this.

Spiders are an evolutionary successful group, with a high number of species adapted to different environments. This diversity of adaptations relies on essential molecules produced by them, which compose venom and silk. Studying the gene structure of these molecules may help to elucidate how spiders have evolved and adapted, disclosing possible mechanisms for generating molecular diversity [ 35 ].

In the present work we have compared the cDNA sequences (derived from mRNA expressed in P. nigriventer venom glands), and the genomic sequences (retrieved from muscular tissue of the spiders’ legs) of three of the main toxins from P. nigriventer venom, in order to elucidate this Ctenidae toxin gene structure. By comparing cDNA and gDNA sequences of toxins PnTx1 and PnTx2-5, both synonymous and non-synonymous point mutations leading to amino acid substitutions were found in the signal peptide, propeptide and in the mature toxin sequence. These nucleotide and amino acid substitutions were expected since spider toxins are known to have many isoforms, forming groups of related sequences differing by point mutations, even within a single spider specimen, so-called combinatorial libraries [ 34 ]. In P. nigriventer , the existence of extensive variation in signal peptide, propeptide and mature sequences for toxins was also already demonstrated [ 4 ]. In addition, these are abundant toxins that are transcribed at a high frequency, which has been associated with elevated mutation rates, so called transcription-associated mutation (TAM) [ 36 ].

The most important finding of the present work is the description, for the first time, of the intronless genomic structure of some important P. nigriventer cysteine-rich peptide toxins. Regarding the presence of introns in toxins genes, 11 spider species have been analyzed to date (including this study) ( Fig. 3 ). All spider genes encoding for cysteine-rich peptide toxins from infra-order Mygalomorphae analyzed so far are intronless [ 15 - 18 ]. Among the Araneomorphae , the gene for a cysteine-rich peptide toxin found in Diguetia canities venom, µ-diguetoxin-Dc1a, has an intron-exon structure [ 14 ] whereas two-domain neurotoxins from Cheiracanthium punctorium and Oxyopes lineatus do not present introns in their toxin gene sequences [ 37 , 38 ], like we have shown in the present report for P. nigriventer toxin genes ( Fig. 3 ).

The analysis of other toxin classes in other Araneomorphae species showed a diverse scenario. Latrotoxins genes were found to be intronless in Latrodectus tridemciguttatus [ 39 ] , but introns were present in these type of toxins genes in Parasteatoda tepidariorum [ 40 ] and Latrodectus hesperus [ 41 ]. Sphingomyelinases D from Loxosceles arizonica [ 42 ] also presented introns in its genes.

To the best of our knowledge, only seven spider genomes have been published to date: Acanthoscurria geniculata (Mygalomorphae) and Stegodyphus mimosarum (Araneomorphae) [ 43 ]; Trichonephila clavipes (Araneomorphae) [ 44 ]; Parasteatoda tepidariorum (Araneomorphae) [ 45 ]; Pardosa pseudoannulata (Araneomorpha) [ 46 ]; Araneus ventricosus (Araneomorphae) [ 47 ] and Dysdera silvatica (Araneomorphae) [ 48 ]. In addition, the 5000 arthropod genome initiative (i5K), which is committed to sequencing and analyzing 5000 high-priority arthropods [ 49 ], made available a draft of Loxosceles reclusa genome. Introns were described as present in all these spider genome analysis, but whether introns are present or not specifically in cystein-rich peptide toxin genes was not informed. As previously mentioned, latrotoxins found in the genome of the common house spider P. tepidariorum did present introns in their gene structure [ 40 ].

To increase the body of information regarding the presence of introns in cysteine-rich peptide toxins, we attempted to use the available spider genomic data to analyze the structure of cysteine-rich peptides in toxin gene sequences. We did not find evidence of introns in the genomic sequences of A. geniculata (Mygalomorphae), corroborating the previous findings in the literature for this group. Regarding Araneomaphae spiders, the in-silico preliminary analysis did not show evidence of intron presence in cysteine-rich peptide toxins in two species ( L. reclusa and D. sylvatica). However, despite most of the toxin gene sequences being apparently intronless, in the other four analyzed species ( A. ventricosus, P. pseudoannulata, S. mimosarum and T. clavipes) the discontinuity of some alignments pointed to the possible presence of introns in a few toxins gene sequences.

Here, our experimental results showed that P. nigriventer do not present introns in the cysteine-rich peptide toxin sequences analyzed, corroborating most of the other experimental and in-silico findings that indicate the absence of introns in this class of spider toxins. However, since in the genomic data analysis we found evidence of the occurrence of introns in some toxin gene sequences from other Araneomorphae spiders, we cannot discard the possibility that other Phoneutria toxins can also present introns in their sequences. For instance, the co-existence of gene copies both with and without introns has been demonstrated for the elongation factor-1α (EF-1α) in Salticidae spiders from the genus Habbronatus [ 50 ].

Pineda et al. [ 18 ], in their work describing the gene structure of toxins from Australian funnel-web spiders, hypothesized that Mygalomorphae spiders had lost their toxin gene introns through their evolutionary history, whereas Araneomorphae kept them from a common ancestor. However, considering that different families of toxins have different features concerning the presence of introns and no consensus could be found among Araneomorphae, the evolutionary history of spider peptide neurotoxins seems to be more complex and remains to be further clarified.

Venom toxins are essential for spider survival and a high level of toxin expression in the venom glands is required. Gene expression increase may occur through the polyploidization of venom gland tissue cells [ 51 ]. Intron loss is another possible mechanism for increasing gene expression, since it has been reported that highly expressed genes tend to lose introns more frequently and gain introns more rarely than genes with low expression levels [ 52 ]. Furthermore, the absence of introns has also been related to an increase of mutation rates [ 53 ], which in turn may contribute to the high variability of toxin sequences and the emergence of new spider toxins [ 38 ]. On the other hand, the presence of introns in toxin genes may modulate the mutation rates of each exon separated by them [ 54 ], constituting a different mechanism for toxin evolution and diversification. Besides that, as most of spider toxin DNA sequences come from venom gland transcriptome analysis , the roles of alternative splicing, gene duplication and other regulatory controls in generating venom molecular diversity still need to be further studied.

Conclusions

In this work, we have investigated the structure of the genes encoding for three known sodium channel modulator toxins from P. nigriventer venom and the results indicate that P. nigriventer toxins do not contain introns in their genes sequences. However, since we also found evidence of toxin genes with and without introns in genomes of other Araneomorphae spiders, we cannot rule out the possibility of the presence of introns in other P. nigriventer toxin genes. This can only be confirmed after sequencing its genome.

As the majority of spider toxin DNA sequences come from venom glands transcriptomes studies, the gene structure of toxins from most spider toxins are still obscure. Thus, we believe that our results can contribute to future studies on understanding the mechanisms underlying spider venom molecular diversity, as well as the evolutionary aspects of spider toxins.

Abbreviations

gDNA: genomic DNA; ICK: inhibitor cysteine knot; PnTx1: μ-ctenitoxin-Pn1a; PnTx2-5: δ-ctenitoxin-Pn2c; PnTx4(5-5): ɣ-ctenitoxin-Pn1a; TAM: transcription-associated mutation.

Availability of data and materials: All data generated or analyzed during this study are included in this article.

Funding: Not applicable.

Ethics approval: The authors declare that the spiders used in this work were collected with approval of the Brazilian Institute of the Environment and Renewable Natural Resources (IBAMA). The project was also registered in National System for Management of Genetic Heritage and Associated Traditional Knowledge (SisGen): #A26E945.

Consent for publication: Not applicable.

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Brazilian Wandering Spider facts

Last updated on July 12th, 2023 at 01:48 pm

When you think of deadly spiders, there are a few names that spring to mind – but none more infamous than the Brazilian Wandering Spider. This species is reputed to have the most toxic venom of any spider, having a bite which causes horrendous side-effects like priapism and convulsions.

What you may not know, however, is that the Brazilian Wandering Spider name is actually used for a few species. The most common, and perhaps the most medically significant in the group are Phoneutria nigriventer and Phoneutria fera .

In this post, I’ll tell you more about these two species, from where they live, to what they eat. To keep things simple, I’ll just refer to them both as the “Brazilian Wandering Spider”, given how similar they are. Let’s dive in…

Quick Facts

To kick things off, here are some fascinating factoids about the Brazilian Wandering Spider:

• They belong to the genus ‘Phoneutria’, which translates to ‘murderess’ in Greek.
• They are known for their highly potent venom.
• Wandering Spiders are nocturnal creatures.
• They are also known as ‘banana spiders’ due to their tendency to hide in banana plants.
• They are not web-weavers but active hunters. This is called ‘cursorial’ hunting.
• It’s considered one of the most dangerous spiders in the world.

Other Common Names

The Brazilian Wandering Spider goes by several other names. The most common is the ‘banana spider’, thanks to their notorious habit of stowing away in banana shipments. In their native Portuguese, they’re known as ‘aranhas-armadeiras’ , translating to ‘armed spiders’ – a reference to their aggressive defense posture.

Brazilian Wandering Spider Venom

Possessing one of the most potent venoms among spiders, the Brazilian Wandering Spider’s bite is a cause for concern. Its venom is a complex cocktail of toxins, proteins, and peptides.

The main component that gets everyone’s attention is the neurotoxin, called PhTx3 , which can interfere with the functioning of our nervous system, leading to a variety of symptoms.

What’s the Benefit of Having Such Strong Venom?

With venom potent enough to kill a human, one might wonder why this spider needs such a powerful weapon. The answer lies in its lifestyle.

Brazilian Wandering Spiders are active hunters and their venom is primarily used to incapacitate prey quickly. The venom’s potency also serves as an effective deterrent against potential predators.

Brazilian Wandering Spider Deaths

Despite the notorious reputation, actual deaths from Brazilian Wandering Spider bites are rare. This is largely due to the rapid medical attention available in areas where these spiders are common. Plus, these spiders don’t always inject venom when they bite – a dry bite can occur.

This actually common in venomous animals, including spiders and reptiles. Occasionally they bite and decide to not inject any venom. The point of this is to conserve it, given that it is energetically costly to produce.

Brazilian Wandering Spider Size

Being quite large and impressive compared to most arachnids, adult Brazilian Wandering Spiders can reach a leg span of up to 7 inches (18 cm) . The body size excluding the legs can be up to 2 inches (5 cm). Their size contributes to their intimidating presence.

If you’d to learn more about why they get so big, check out my article on Brazilian Wandering Spider size for more info!

Brazilian Wandering Spider Location and Habitat

Brazilian Wandering Spiders are native to South America. Here’s a quick rundown of their range and preferred habitats:

Brazilian Wandering Spider Speed

An adept hunter, the Brazilian Wandering Spider can move quickly when chasing prey or evading threats. While exact speed measurements can vary, some sources report that these spiders can achieve speeds up to 1 meter per second.

What Does the Brazilian Wandering Spider Eat?

The Brazilian Wandering Spider’s diet consists mainly of insects, other spiders, and occasionally small amphibians and reptiles. Their potent venom allows them to tackle prey larger than themselves, making them one of the apex micro-predators in their habitat.

Final Thoughts…

While the Brazilian Wandering Spider might seem terrifying to many, as an arachnid enthusiast, I find them to be incredibly fascinating. Their potent venom, hunting prowess, and adaptation to diverse habitats reveal the intricate beauty of the evolutionary process.

Just remember, these spiders, like all creatures, play a vital role in our ecosystem. Respect, not fear, should be our response to these remarkable arachnids.

The truth is that most bites are accidental, but they do occur. The fact that so few deaths occur each year is a testament to the effectiveness of the antivenom that has now become widely available.

FAQ related to the Brazilian Wandering Spider

What happens if you are bitten by a brazilian wandering spider.

Immediately after a bite from a Brazilian Wandering Spider, you will experience localized pain. Then, within 5 to 15 minutes the area around the bite will swell. The swelling can spread to most of a limb, for example. Finally, neurological symptoms like coldness, sweating, and convulsions will set in.

Do wandering spiders jump?

Wandering Spiders are excellent at jumping. They can jump several feet when surprised, and may occasionally use this as a tactic to evade predators. Jumping at you isn’t part of how bites happen though. When faced with humans, Wandering Spiders usually stand their ground and use their threat display of raised legs to warn us away.

Is Brazilian wandering spider friendly?

Wandering Spiders are not friendly. As a general rule, they are relatively calm, but can also be defensive. They tend to see humans as a threat, and will not take to being handled easily. That said, they are not aggressive, and most bites happen when someone accidentally touches one or tries to kill it.

Are Brazilian wandering spiders in Australia?

Brazilian Wandering Spiders are not found in Australia. The only species in Australia that get as large as Wandering Spiders are the Huntsman Spiders. At a distance they may appear relatively similar, but Huntsman Spiders are completely harmless to humans.

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This is one of the most dangerous spider species in the world.

Image Credit: Dr Morley Read/Shutterstock

Meet the Brazilian wandering spiders in the genus Phoneutria , also referred to as the armed spiders or the banana spiders. The members of this group have one of the most dangerous bites of any spider species on Earth and they have been classified as the world’s most venomous spider many times.

Where Do Brazilian Wandering Spiders Live?

Though currently the Guinness World Record is shared with another species – the Sydney funnel-web, ( Atrax robustus ) – the Brazilian wandering spiders are a force to be reckoned with. 

As the name suggests all the Brazilian wandering spiders can be found in northern South America, with at least one species having distributions across more than just Brazil, writes the University of Florida . However, Phoneutria fera and Phoneutria nigriventer are the main species that feature in news articles as they often travel overseas in shipments of bananas, causing panic and confusion – and even the closure of supermarkets . 

What Is The Size Of A Brazilian Wandering Spider?

The size of the Brazilian wandering spiders varies between species. Some can be very large with a leg span of 18 centimeters (7.1 inches). The spiders are famously quite aggressive, and instead of running from threats will rear up onto their back legs and stand their ground. 

What Are The Effects Of A Brazilian Wandering Spider Bite?

Brazilian wandering spiders often make the news because of the unusual symptoms caused to humans after being bitten by one. 

A 2023 study in Frontiers In Molecular Biosciences suggested there were around 4,000 cases of people being bitten by Phoneutria nigriventer in Brazil each year. Their venom affects the nervous system, causing double vision, salvation, irregular heartbeat – and even prolonged painful erections, known as priapism . In fact, because of this, their venom is being explored as a possible treatment for erectile dysfunction conditions.

Can A Brazilian Wandering Spider Bite Kill You?

The neurotoxin PhTx3 is to blame for these symptoms, and according to the Independent , a wandering spider bite can reportedly be fatal in as little as 60 minutes. However, an effective anti-venom is usually enough to save those affected. A study into Phoneutria boliviensis in 2019 suggested that the species had adapted its venom to catch vertebrates as opposed to lizards and amphibians, which might explain why the venom is so toxic to humans. 

It comes as no surprise then that their scientific name Phoneutria means “murderess” in Greek. 

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Brazilian Wandering Spider

Brazilian Wandering Spider , scientifically known as Phoneutria, emerges as a captivating enigma in the realm of arachnids.

Renowned for its formidable reputation as one of the world’s most venomous spiders , Phoneutria embodies a plethora of intriguing traits that have captured the curiosity of enthusiasts and researchers alike.

From its distinctive appearance and neurotoxic venom to its nomadic hunting strategies and unique mating behaviors , this remarkable spider species holds a wealth of fascinating secrets waiting to be unraveled.

Join us as we embark on a journey to explore the captivating world of the Brazilian Wandering Spider, shedding light on its captivating characteristics and dispelling myths that have shrouded its true nature.

1. Taxonomy and Distribution of the Brazilian Wandering Spider

A. scientific classification of phoneutria.

The Brazilian Wandering Spider, scientifically referred to as Phoneutria, occupies a distinct place within the arachnid taxonomy.

Belonging to the family Ctenidae, this spider genus is further categorized into several species, each boasting unique traits and behaviors .

Phoneutria’s taxonomic position not only distinguishes it from its arachnid counterparts but also underscores its intriguing evolutionary journey.

B. Native Habitat in South and Central America

Endemic to the lush landscapes of South and Central America, the Brazilian Wandering Spider finds its natural haven within these diverse regions.

From the rainforests of the Amazon to the tropical stretches of the Caribbean, Phoneutria has adapted to a range of environments over the course of its evolution.

The spider’s ancestral ties to these regions are tightly woven into their behaviors , anatomy, and survival strategies.

C. Preference for Tropical Rainforests and Urban Areas

Within its native territories, the Brazilian Wandering Spider exhibits remarkable versatility in its chosen habitats.

While it thrives amidst the vibrant biodiversity of tropical rainforests, it has also displayed a propensity for urban locales.

Phoneutria’s adaptability has led it to establish a presence in urban areas, where it often finds shelter in crevices, gardens, and even human dwellings.

This adaptability to both wild and urban spaces further showcases the spider’s resilience and capacity to thrive in varying conditions.

2. Physical Characteristics of the Brazilian Wandering Spider

A. size, coloration, and distinctive markings.

The Brazilian Wandering Spider , a creature of remarkable visual intrigue, boasts an array of captivating physical attributes.

Ranging in size from a few centimeters to several inches, Phoneutria showcases a size diversity that reflects the breadth of its genus.

Its coloration varies across species, encompassing shades of brown, black, and gray, often accompanied by intricate patterns and markings that adorn its exoskeleton.

These unique markings serve not only as a visual spectacle but also as essential components of its survival toolkit.

B. Camouflage and Defense Mechanisms

The Brazilian Wandering Spider’s appearance is a masterpiece of evolution, meticulously crafted to ensure both survival and predation .

Its coloration and markings are tailor-made for blending seamlessly into its surroundings, granting it a potent advantage in ambushing prey and evading predators . Moreover, these markings also play a role in its defense mechanisms.

When threatened, Phoneutria adopts a defensive posture, raising its front legs and revealing its striking markings, a visual warning to potential threats. This dual-purpose camouflage and defense strategy exemplify nature’s ingenuity at its finest.

C. Sexual Dimorphism: Unveiling Gender Differences

A fascinating facet of the Brazilian Wandering Spider lies in the realm of sexual dimorphism , where gender-based variations manifest in pronounced ways.

Females tend to be larger and more robust than their male counterparts, showcasing a size disparity that has evolved in tandem with their roles in reproduction and hunting .

Beyond size, other characteristics, such as leg structure and coloration, also exhibit subtle differences between male and female Phoneutria specimens.

This divergence in physical traits adds depth to our understanding of the species’ intricate biology and behavior .

In exploring the physical characteristics of the Brazilian Wandering Spider , we uncover a canvas painted with size diversity, intricate coloration, and unique markings.

These features, finely tuned by evolution, contribute to its prowess in camouflage and defense, while the fascinating interplay of sexual dimorphism further enriches our perception of this captivating arachnid species .

3. Venomous Nature of the Brazilian Wandering Spider

A. potent neurotoxic venom: a silent lethal weapon.

The Brazilian Wandering Spider, known scientifically as Phoneutria, harbors a venomous arsenal that stands as a testament to nature’s intricate design.

This spider’s venom contains a potent concoction of neurotoxic compounds, tailored by evolution to incapacitate its prey swiftly and efficiently.

The neurotoxins interfere with nerve cell communication, leading to paralysis and ensuring that Phoneutria’s quarry is rendered immobile and defenseless, setting the stage for a successful meal.

B. Effects on Prey and Human Hazard

When a victim succumbs to the Brazilian Wandering Spider’s venom , the effects are a symphony of paralysis and predation .

The venom’s impact on the prey’s nervous system results in swift immobilization, offering the spider a decisive advantage in subduing its catch.

While this venomous efficiency is well-adapted for predation, it also underscores the potential danger to humans.

A bite from Phoneutria can lead to a series of neurotoxic reactions, with varying degrees of severity depending on factors such as the individual’s age and overall health.

While human envenomations are relatively rare, they can result in a range of symptoms, from localized pain and swelling to more severe neurological effects.

C. Recorded Cases of Envenomations: Unraveling the Symptoms

Throughout history, documented cases of Phoneutria envenomations have offered insights into the spider’s potential threat to humans .

Symptoms typically include intense pain at the bite site, accompanied by swelling and redness . In some instances, victims have reported systemic reactions, such as muscle cramps, elevated heart rate, and even breathing difficulties.

Swift medical attention and the administration of antivenom have proven effective in mitigating the severity of these symptoms.

These cases serve as a reminder of the delicate balance between the Brazilian Wandering Spider’s potent venom and the potential risks it poses to those who unwittingly encounter it.

4. Hunting and Diet of the Brazilian Wandering Spider

A. hunting techniques and wandering behavior.

The Brazilian Wandering Spider, scientifically known as Phoneutria, unveils a mesmerizing repertoire of hunting techniques that set it apart as a master predator .

Displaying an agile and nomadic behavior , Phoneutria does not confine itself to the confines of a web. Instead, it actively prowls its surroundings, tirelessly searching for potential prey.

This dynamic wandering behavior ensures that its chances of encountering a variety of food sources are maximized, showcasing a strategic approach to sustenance.

B. Active Hunting Triumphs Over Web-Building

Unlike its web-weaving counterparts, the Brazilian Wandering Spider relies on a more hands-on approach to securing its next meal.

While weaving webs might seem an efficient method, Phoneutria’s active hunting strategy offers a distinct advantage in versatility.

By forgoing the constraints of a stationary web, it can tailor its approach to suit different environments and prey types, adapting its tactics on the fly.

This adaptability demonstrates the spider’s remarkable ability to adjust its methods for optimal results.

C. Diverse Prey Spectrum: Insects to Small Vertebrates

Phoneutria’s diet is a testament to its prowess as an opportunistic predator . Its menu spans a diverse range of creatures, from insects like crickets and cockroaches to small vertebrates such as lizards and frogs , and even small rodents.

This wide-ranging palate highlights its ecological significance in controlling various populations within its habitat.

By consuming creatures both large and small, Phoneutria ensures a balanced ecosystem, playing a crucial role in maintaining biodiversity and ecological equilibrium.

5. Mating and Reproduction of the Brazilian Wandering Spider

A. courtship rituals and behaviors: a complex affair.

The Brazilian Wandering Spider, scientifically referred to as Phoneutria, reveals a captivating array of courtship rituals and behaviors that form the cornerstone of its reproductive cycle.

Courtship among these arachnids is a complex affair, involving intricate dances and displays that serve as both communication and assessment.

Male Phoneutria employs a combination of visual cues, vibrations, and tactile interactions to court potential mates.

This elaborate courtship process highlights the significance of precise communication in the delicate dance of reproduction .

B. Cannibalistic Tendencies: A Post-Mating Phenomenon

An aspect that sets Phoneutria’s mating process apart is the notorious cannibalistic tendency exhibited by females after mating.

Following successful mating, females may exhibit an inclination to consume their partners. This seemingly counterintuitive behavior has evolutionary underpinnings.

It is believed that this cannibalistic act not only provides the female with a much-needed nutritional boost but also eliminates potential competitors and safeguards the male’s investment in the next generation.

This intriguing behavior sheds light on the complexities of reproductive strategies within the species.

C. The Unique Mating Plug Phenomenon: A Puzzling Enigma

A distinctive feature in Phoneutria’s reproductive saga is the enigmatic mating plug phenomenon. After mating, male Phoneutria deposit a specialized substance that forms a plug within the female’s reproductive tract.

This plug is believed to serve multiple purposes. It may prevent other males from mating with the female, thus ensuring the successful transmission of the mating male’s genetic material.

Additionally, it might aid in sealing off the female’s reproductive tract, potentially protecting her from external pathogens.

This phenomenon underscores the intricate interplay of biological strategies that contribute to the species’ reproductive success.

6. Human Interaction and Urban Legends of the Brazilian Wandering Spider

A. occasional presence in urban areas: nature in our midst.

The Brazilian Wandering Spider, scientifically known as Phoneutria, has carved a niche for itself not only in the wild but also in the fabric of urban environments.

While its primary habitats are the lush landscapes of South and Central America, Phoneutria occasionally ventures into human -inhabited spaces. Its adaptability allows it to find shelter in gardens, crevices, and even within homes.

This coexistence with humans adds an intriguing dimension to our encounters with this enigmatic arachnid .

B. Debunking Misconceptions: Separating Fact from Fiction

The presence of the Brazilian Wandering Spider has sparked a plethora of misconceptions and exaggerated tales, contributing to the creation of urban legends.

Stories of spiders leaping from banana bunches or hiding under toilet seats have become part of modern folklore, often fueled by sensationalism.

It’s crucial to sift through these tales and recognize that while Phoneutria’s venom is potent, the likelihood of encountering a dangerous encounter is relatively low.

Separating fact from fiction empowers individuals to approach these creatures with accurate knowledge.

C. Importance of Proper Education: Identifying Friend from Foe

Education plays a pivotal role in fostering a harmonious coexistence between humans and the Brazilian Wandering Spider .

Learning to identify and understand the behaviors of Phoneutria species enhances safety for both humans and the spiders themselves.

Instead of succumbing to unwarranted fear, individuals can take steps to reduce the chances of accidental encounters and, if necessary, engage in responsible removal methods.

By arming themselves with knowledge, individuals can navigate encounters with urban-dwelling Phoneutria specimens with confidence and respect.

7. Brazilian Wandering Spider Conservation and Misunderstanding

A. significance of phoneutria in ecosystem dynamics.

The Brazilian Wandering Spider , scientifically termed Phoneutria, assumes a pivotal role within its ecosystem, contributing to a delicate balance of populations and interactions.

As a top-tier predator , it plays a crucial part in controlling insect and small vertebrate populations, preventing unchecked growth that could disrupt the ecosystem’s equilibrium.

By maintaining these population dynamics, Phoneutria ensures the health and stability of its habitat, highlighting its significance beyond its ominous reputation.

B. Impact of Fear and Misunderstanding: Hindrances to Conservation

Despite its ecological contributions, the Brazilian Wandering Spider often falls victim to fear-driven misconceptions that negatively impact conservation efforts.

Misunderstandings surrounding its behavior and potential danger can lead to unwarranted extermination campaigns and habitat destruction.

Fear-driven reactions not only disrupt the natural balance but also hinder opportunities to study and appreciate the species for its ecological significance.

Addressing these misconceptions is crucial to ensuring the spider’s survival and maintaining the health of its ecosystems.

C. Efforts to Dispel Myths and Promote Coexistence

Efforts to conserve the Brazilian Wandering Spider are interwoven with endeavors to educate and dispel myths.

By providing accurate information and dispelling exaggerated tales, conservationists aim to reshape public perception.

Collaborative initiatives emphasize coexistence, highlighting the importance of responsible behavior when encountering Phoneutria.

Educating communities about the spider’s role, behavior, and conservation status fosters an environment where fear gives way to appreciation, and where balanced cohabitation becomes a reality.

8. Research and Medical Significance of the Brazilian Wandering Spider

A. ongoing scientific research on phoneutria venom.

The Brazilian Wandering Spider , Phoneutria, has garnered significant attention from the scientific community due to the unique properties of its venom.

Ongoing research delves into the intricate composition of the venom, aiming to unlock its mysteries and potential applications in various fields.

The diverse array of compounds within the venom, particularly its neurotoxic components, has attracted interest for their potential medical and therapeutic implications.

B. Antivenom Development and Therapeutic Prospects

One of the most promising areas of research surrounding Phoneutria lies in the development of antivenoms and therapeutic agents.

The venom’s potent neurotoxic effects on the nervous system have spurred efforts to create targeted treatments for conditions such as chronic pain and neurological disorders .

Additionally, the potential for antivenoms holds promise in mitigating the effects of envenomations, offering a lifeline for individuals who encounter these spiders .

This focus on harnessing the venom’s properties for positive medical outcomes highlights the transformative potential within this enigmatic arachnid .

C. Balanced Perspectives: Navigating Ethical and Scientific Endeavors

While research on the Brazilian Wandering Spider’s venom offers tremendous potential, it necessitates a balanced perspective.

As researchers probe the venom’s properties, ethical considerations arise, including the well-being of the spiders and their ecosystems.

A holistic approach acknowledges the value of understanding Phoneutria’s natural behaviors and conserving its habitats.

This balanced perspective extends to utilizing the venom’s potential responsibly, ensuring that breakthroughs are achieved while respecting the complex interplay of science and nature.

9. Frequently Asked Questions about the Brazilian Wandering Spider

What is the brazilian wandering spider.

The Brazilian Wandering Spider , scientifically known as Phoneutria, is a venomous arachnid found in South and Central America. It’s notorious for its potent venom and is considered one of the most venomous spiders in the world.

Is the Brazilian Wandering Spider dangerous to humans?

Yes, the Brazilian Wandering Spider’s venom contains potent neurotoxins that can cause a range of symptoms in humans , from localized pain and swelling to more severe reactions. While bites are relatively rare, it’s advisable to exercise caution when encountering these spiders.

What is the spider’s habitat?

The Brazilian Wandering Spider is native to tropical rainforests of South and Central America. However, it’s adaptable and can also be found in urban areas, such as gardens and houses.

How does the Brazilian Wandering Spider hunt?

Unlike many spiders that build webs, Phoneutria is an active hunter. It roams its environment in search of prey, relying on its keen senses to detect vibrations and movements.

Are Brazilian Wandering Spiders aggressive toward humans?

Brazilian Wandering Spiders are not naturally aggressive towards humans and will typically only bite in self-defense. However, caution is advised, especially in areas where these spiders are known to inhabit.

Can the Brazilian Wandering Spider’s venom be used for medical purposes?

Yes, research is ongoing into the potential medical applications of Phoneutria’s venom. Its neurotoxic properties have sparked interest in pain management and neurological treatments.

Is the spider’s reputation for crawling into banana shipments true?

While there have been stories of Brazilian Wandering Spiders being found in shipments of bananas, these occurrences are extremely rare. Spiders are unlikely to survive the conditions of shipping and storage.

How can I stay safe around Brazilian Wandering Spiders?

To stay safe, it’s important to be cautious when encountering spiders in their natural habitat. Avoid provoking or handling them, especially if you’re unsure of their identity. If you suspect you’ve been bitten, seek medical attention promptly.

Are there any efforts to conserve the Brazilian Wandering Spider?

Conservation efforts for the Brazilian Wandering Spider are intertwined with public education and dispelling myths. Recognizing its role in ecosystems and promoting coexistence are essential steps in preserving this unique species.

What can I do if I find a Brazilian Wandering Spider in my home?

If you encounter a Brazilian Wandering Spider in your home, it’s advisable to contact local pest control professionals who can safely remove the spider without causing harm.

In the intricate tapestry of nature, the Brazilian Wandering Spider , Phoneutria, emerges as a creature of both fascination and caution.

Its venomous nature and captivating behaviors have earned it a place among the most enigmatic arachnids .

As we continue to explore its world, debunk myths, and understand its vital role in ecosystems, we find a delicate balance between awe and respect.

Armed with knowledge, we navigate the realm of Phoneutria, appreciating its complexity while fostering coexistence, a testament to the intricate dance between humans and the natural world.

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The brazilian wandering spider: threats and treatments.

Imagine being bitten by a spider that not only causes excruciating pain, but also induces a painful and prolonged erection. This peculiar arachnid is no other than the Brazilian Wandering Spider, a creature that strikes fear into the hearts of many. This article explores the threats posed by this venomous spider and examines the treatments available for its bite. Prepare to discover the terrifying world of the Brazilian Wandering Spider and the measures taken to combat its deadly effects.

Table of Contents

Overview of the Brazilian Wandering Spider

Introduction to the Brazilian Wandering Spider

The Brazilian Wandering Spider, scientifically known as Phoneutria, is a species of venomous spider found primarily in South and Central America, with Brazil being its native habitat. These spiders are known for their distinct hunting techniques, potent venom, and the ability to wander, hence their name. With its aggressive nature and potentially lethal bite, the Brazilian Wandering Spider poses a significant threat to humans and animals alike.

Preferred Habitat and Distribution

Brazilian Wandering Spiders are adaptable creatures that can thrive in various habitats, ranging from tropical rainforests to urban areas. They prefer dark and secluded areas like tree stumps, piles of leaves, and crevices, typically found in the wilderness. However, due to deforestation and human encroachment, these spiders have also adapted to urban environments, often found hiding in woodpiles, sheds, and even inside homes.

Physical Characteristics

The Brazilian Wandering Spider is a large arachnid, with a leg span that can reach up to 6 inches. They have a brownish appearance with darker markings, aiding in their camouflage amidst bark and leaves. These spiders possess strong legs, enabling them to move quickly and gracefully. The most distinguishing feature of the Brazilian Wandering Spider is their characteristic defensive posture, where they raise their front legs to display their fangs.

Behavior and Hunting Techniques

Unlike most spiders that build webs for hunting, Brazilian Wandering Spiders are active hunters. They spend their nights on the move, searching for prey and avoiding potential predators. These spiders have excellent eyesight and rely on their acute senses to detect movements and vibrations. When hunting, they employ a unique technique known as “lurking,” where they stay hidden, waiting for their prey to approach before quickly pouncing on it.

Diet of Brazilian Wandering Spiders

Brazilian Wandering Spiders have a varied diet, which includes insects, small rodents, and even lizards. Their venomous bite immobilizes their prey, making it easier for the spider to handle and consume. While they primarily feed on live prey, these versatile spiders can also scavenge for food when necessary, increasing their chances of survival in harsh environments.

Venomous Threats Posed by Brazilian Wandering Spiders

Potency of brazilian wandering spider venom.

The venom of the Brazilian Wandering Spider is considered one of the most potent among spider species. It contains a neurotoxin called PhTx3, which affects the nervous system, causing severe pain, muscle spasms, and potentially life-threatening reactions. Due to its toxicity, the venom of this spider is not only harmful to humans but can also be lethal to animals, including pets.

Signs and Symptoms of Spider Bites

When bitten by a Brazilian Wandering Spider, the symptoms can vary depending on the individual’s sensitivity and the amount of venom injected. Common signs of spider bites include intense pain, redness, swelling, and local tissue damage. In severe cases, individuals may experience systemic effects like muscle cramps, increased heart rate, sweating, and even difficulty breathing. It is crucial to seek medical attention immediately if bitten by this spider.

Health Risks and Potential Complications

Being bitten by a Brazilian Wandering Spider can lead to various health risks and potential complications. The neurotoxic effects of the venom can cause paralysis, respiratory failure, and in extreme cases, death. Additionally, some individuals may develop allergic reactions to the venom, further exacerbating the severity of the bite. Prompt medical treatment is essential to minimize the risks and complications associated with these spider bites.

Comparison to other Venomous Spiders

Compared to other venomous spiders, such as the Black Widow or Brown Recluse, the Brazilian Wandering Spider’s bite is known to be more lethal due to its potent venom. While the Black Widow and Brown Recluse spiders are found in different regions, their venom can cause severe local tissue damage and systemic symptoms as well. Each spider species presents unique risks, and understanding their differences is crucial in providing appropriate medical treatment.

Fatalities and Incidents Reported

Fatalities related to Brazilian Wandering Spider bites are rare, thanks to the availability of antivenom and timely medical interventions. However, incidents of spider bites resulting in immediate hospitalization and severe complications have been documented. Particularly vulnerable to these bites are children, the elderly, and those with underlying health conditions. Prompt reporting and proper management of spider bites are crucial to prevent tragic outcomes.

Medical Treatments for Brazilian Wandering Spider Bites

Emergency response and first aid measures.

In the event of a Brazilian Wandering Spider bite, immediate action is necessary. First and foremost, it is essential to stay calm and seek medical help without delay. While waiting for medical professionals to arrive, follow basic first aid measures, including cleaning the wound with mild soap and water, applying a cold compress to reduce swelling, and keeping the affected limb immobilized to minimize venom spread.

Antivenom Administration for Spider Bites

Antivenom is the primary treatment for Brazilian Wandering Spider bites. It contains antibodies that neutralize the venom’s effects, reducing pain and preventing further complications. Medical professionals will carefully administer the antivenom, closely monitoring the patient’s vital signs and ensuring appropriate dosage. Antivenom therapy is vital in counteracting the potent neurotoxin and providing the best chance for a successful recovery.

Management of Pain and Swelling

To alleviate pain and reduce swelling associated with spider bites, various medications can be prescribed. Nonsteroidal anti-inflammatory drugs (NSAIDs) are commonly used to decrease inflammation and relieve discomfort. In some cases, opioids may be necessary to manage severe pain. Applying ice packs to the bite site and elevating the affected limb can also help reduce swelling.

Treatment Approaches for Systemic Effects

When systemic effects occur due to a Brazilian Wandering Spider bite, additional treatment approaches are required. Muscle relaxants and anticonvulsant medications may be administered to control muscle spasms and reduce the risk of convulsions. Supportive care, such as intravenous fluids and oxygen therapy, may also be provided to maintain hydration and ensure respiratory stability.

Long-Term Effects and Follow-Up Care

Even after the initial treatment of a Brazilian Wandering Spider bite, long-term effects may persist. Some individuals may experience residual pain, muscle weakness, or psychological trauma. Follow-up care is vital to monitor and manage any lingering symptoms. Physical therapy and counseling may be recommended to aid in rehabilitation and promote psychological well-being.

Prevention and Control Measures

Understanding spider behavior and habitats.

Understanding the behavior and habitats of Brazilian Wandering Spiders is crucial for effective prevention and control. Being aware of their preference for dark, secluded areas allows individuals to take proactive measures to minimize encounters. Regularly inspecting and cleaning potential hiding spots and sealing any gaps or cracks in homes and buildings can significantly reduce the chances of a spider infestation.

Spider Bite Prevention Tips

To prevent spider bites, adopting certain preventive measures is advisable. Avoid reaching into dark spaces without proper visibility, especially when gardening or working with woodpiles. Shake out clothing and shoes before wearing them, as spiders may seek refuge in these items. Using gloves when handling items in potential spider habitats can also provide a line of defense against accidental bites.

Safety Measures for Homes and Buildings

Creating a spider-free environment within homes and buildings can be achieved through implementing safety measures. Regularly clean and declutter living spaces, as spiders are attracted to dark and undisturbed areas. Installing screens on windows and doorways can prevent spiders from entering, and using weatherstripping to seal gaps will minimize entry points. Additionally, keeping outdoor lights off or changing the color to be less attractive to insects can also deter spiders.

Protective Clothing and Gear

When venturing into areas known for spider activity, wearing protective clothing and gear is essential to minimize the risk of bites. Long-sleeved shirts, long pants, and closed-toe shoes can provide a physical barrier between the spider and the skin. Additionally, using gloves, hats, and face shields can further protect vulnerable areas, reducing the chances of accidental bites.

Insecticides and Pest Control Methods

In cases where spider infestations become significant and pose a threat, the use of insecticides and professional pest control methods may be necessary. It is important to follow local regulations and recommendations when applying insecticides, as some may be harmful to humans and pets. Seeking the assistance of licensed pest control experts ensures effective treatment while prioritizing safety.

Research and Studies on Brazilian Wandering Spiders

Scientific studies and species classification.

Scientific studies play a crucial role in expanding our knowledge of Brazilian Wandering Spiders. Researchers conduct studies to better understand their taxonomy, behavior, and venom composition. Species classification helps identify specific variations within the Phoneutria genus, allowing for more targeted research and providing a foundation for conservation efforts.

Venom Extraction and Composition

Extracting and analyzing the venom of Brazilian Wandering Spiders is essential for developing effective antivenom and understanding the biochemical properties of the venom. Researchers aim to identify the specific toxins present, their mechanisms of action, and potential therapeutic applications. Studying venom composition can uncover valuable insights into the spider’s hunting strategies and aid in the development of novel pharmaceuticals.

Antivenom Development and Efficacy

Research on antivenom development focuses on improving the efficacy and safety of existing treatments. Scientists work to refine antivenom formulations, ensuring they neutralize the spider’s venom effectively. Testing the antivenom’s efficacy against various species of Brazilian Wandering Spiders is crucial to provide broad coverage and maximize the chances of successful treatment.

Environmental Impact and Conservation

Understanding the environmental impact of Brazilian Wandering Spiders is essential for effective conservation strategies. Research delves into the spider’s role in the ecosystem, its interactions with other species, and the potential consequences of population decline. By assessing their conservation status and identifying threats, scientists and policymakers can develop measures to protect this species and preserve its natural habitats.

Future Research Directions

As scientific advancements continue, future research on Brazilian Wandering Spiders will focus on areas such as genetic studies, venom evolution, and behavior analysis. Deepening our understanding of their genetics can provide insights into their adaptability and evolutionary history, aiding in conservation efforts. Additionally, studying behavioral patterns can enhance our ability to predict their movements and prevent human encounters.

Emerging Concerns and Cases of Brazilian Wandering Spider

Global spread and entry into new regions.

The global spread of the Brazilian Wandering Spider is a growing concern. Due to international trade and transportation, these spiders have been inadvertently introduced to regions outside their native habitat. Their ability to adapt to new environments increases the risk of establishing invasive populations, impacting local ecosystems and potentially posing a threat to human health.

Importance of Surveillance and Reporting

Surveillance and reporting systems are vital in monitoring and preventing the spread of Brazilian Wandering Spiders. Prompt and accurate reporting of potential sightings ensures swift action can be taken to mitigate the risks associated with these spiders. Encouraging public involvement and providing education on spider identification can help improve surveillance efforts and enable effective measures to be implemented.

Documented Cases Outside Brazil

While the Brazilian Wandering Spider is primarily found in Brazil, documented cases of encounters and bites have been reported in other countries. This highlights the potential for these spiders to establish populations beyond their native range and emphasizes the need for international cooperation in addressing this emerging concern. Sharing knowledge and experiences across borders is crucial in managing and preventing spider-related incidents.

Impact on Tourism and International Trade

The presence of Brazilian Wandering Spiders in areas heavily reliant on tourism and international trade can have significant economic implications. Fear of spider encounters and bites may deter tourists and affect the tourism industry. Moreover, the risk of transporting spiders through international trade, particularly in goods such as fruits or plants, poses a biosecurity concern that requires strict monitoring and prevention measures.

Legal and Regulatory Measures

To address the emerging concerns associated with the Brazilian Wandering Spider, legal and regulatory measures are necessary. Countries need to establish and enforce regulations on the import and export of potentially infested goods, ensuring adequate inspection protocols are in place. Collaboration between governments, organizations, and industries is crucial in establishing effective policies to minimize the risks posed by these spiders.

Interactions and Reactions from Local Communities

Fear and anxiety-related reactions.

The presence of Brazilian Wandering Spiders often elicits fear and anxiety among local communities. The aggressive behavior and potential dangers associated with these spiders contribute to a negative perception. Understanding the psychological impact of these fears is essential to develop educational programs and support services that address community concerns and promote emotional well-being.

Education and Awareness Programs

Educational initiatives and awareness programs play a vital role in mitigating the fears surrounding Brazilian Wandering Spiders. Providing accurate information about the spiders, their behavior, and the appropriate actions to take in case of encounters or bites can help alleviate anxiety and empower individuals to respond effectively. School programs, community workshops, and online resources are valuable tools to disseminate information and promote awareness.

Myths, Folklore, and Cultural Beliefs

Brazilian Wandering Spiders often find themselves entwined in myths, folklore, and cultural beliefs. Some local communities associate these spiders with superstitions and consider them to be omens or symbols of danger. Understanding these cultural beliefs and engaging in respectful dialogue is crucial to dispel myths, foster a better understanding of the spiders, and promote a harmonious coexistence.

Spider as a Symbol in Art and Media

The intriguing nature of the Brazilian Wandering Spider makes it a subject of fascination in art, literature, and media. Artists incorporate the spider’s image into various forms of expression, creating artwork that captures its mystique and intricate details. It serves as a reminder of the spider’s significance in both natural and cultural contexts, sparking conversations and encouraging further exploration.

Local Efforts for Spider Conservation

Communities residing in areas populated by Brazilian Wandering Spiders often play a crucial role in their conservation. Local conservation efforts may involve initiatives such as promoting sustainable land use, raising awareness about the importance of biodiversity, and establishing protected areas or nature reserves. Engaging local communities in spider conservation fosters a sense of ownership and shared responsibility for safeguarding these fascinating creatures.

Comparison with Other Dangerous Spider Species

Brown Recluse Spider

The Brazilian Wandering Spider and the Brown Recluse Spider both pose threats to humans, but they have distinct characteristics. While the Brazilian Wandering Spider is known for its wandering nature and highly potent neurotoxic venom, the Brown Recluse Spider is recognized for its reclusive behavior and venom that can cause necrotic skin lesions. Understanding the differences between these species is crucial in providing appropriate medical treatment for bites.

Black Widow Spider

Both the Brazilian Wandering Spider and the Black Widow Spider are venomous, but their venom composition and effects differ. While the Brazilian Wandering Spider’s venom primarily affects the nervous system, the Black Widow Spider’s venom contains neurotoxins that target the neuromuscular junctions. Recognizing the symptoms and seeking appropriate medical treatment are essential in managing bites from these spiders.

Sydney Funnel-Web Spider

The Brazilian Wandering Spider and the Sydney Funnel-Web Spider are both known for their potent venom and aggressive behavior. However, the Sydney Funnel-Web Spider is native to Australia, while the Brazilian Wandering Spider is found in South and Central America. Despite their geographical differences, both spiders require urgent medical attention in the case of bites due to the potential severity of their venom.

Redback Spider

The Redback Spider, native to Australia, is similar to the Brazilian Wandering Spider in terms of venom potency and potentially lethal bites. They both belong to the family of spiders known for their neurotoxic venom. While the Brazilian Wandering Spider is more active and known for its wandering behavior, the Redback Spider tends to build webs and wait for their prey. Understanding their distinct characteristics is essential in providing targeted medical treatment.

Taipan Spider

The Taipan Spider, also known as the Coastal Taipan or Australian Tarantula, is another venomous spider species found in Australia. Its unique venom composition makes it distinct from the Brazilian Wandering Spider. The Taipan Spider is known for its highly potent neurotoxic venom, but its behavior and physical characteristics differ significantly from those of the Brazilian Wandering Spider. Recognizing the differences helps in accurately identifying and managing spider encounters.

Misidentification and Popular Misconceptions

Confusion with harmless spider species.

Spider misidentification is a common occurrence, leading to unnecessary panic and fear. The Brazilian Wandering Spider may be mistaken for non-venomous species, such as harmless Huntsman or Wolf spiders, due to a superficial resemblance. Educating the public about distinguishing features and encouraging accurate identification can prevent unnecessary concern and promote a better understanding of these spiders.

Spider Hoax and Urban Legends

Urban legends and hoaxes involving spiders, including the Brazilian Wandering Spider, have contributed to public misconceptions. Sensationalized stories on social media or unreliable sources often exaggerate the dangers associated with these spiders, perpetuating unnecessary fears. Encouraging critical thinking and relying on reputable sources for information can help dispel myths and prevent the spread of false information.

Exaggerated Claims and Sensationalism

Exaggerated claims and sensationalism in media portrayals of the Brazilian Wandering Spider can contribute to public hysteria. Highlighting the spiders’ aggressive behavior and potential lethality without providing accurate context can create unnecessary panic. Promoting responsible journalism that presents factual information and provides balanced perspectives is essential to ensure accurate public understanding of these spiders.

Social Media Impact on Public Perception

Social media platforms have a significant influence on public perception and understanding of the Brazilian Wandering Spider. Misinformation can spread rapidly, contributing to fear and misunderstanding. It is crucial to encourage responsible sharing of information, verify facts before sharing, and promote scientific literacy to combat the spread of inaccuracies and ensure accurate portrayals of these spiders.

Expert Clarifications and Reliable Sources

Experts and reliable sources play a vital role in clarifying misconceptions surrounding the Brazilian Wandering Spider. Educating the public about the spiders’ behavior, venom potency, and risks through reputable sources is crucial. Scientists, researchers, and medical professionals can provide accurate and evidence-based information, addressing concerns and dispelling myths surrounding these spiders.

Conservation Efforts and Habitat Protection

Need for conservation initiatives.

Conservation initiatives are crucial to protect the Brazilian Wandering Spider and its natural habitats. The preservation of biodiversity, including these unique arachnids, is essential for maintaining healthy ecosystems. By recognizing the ecological importance of spiders and their role in pest control, conservation efforts can be integrated into broader strategies aimed at preserving the planet’s biodiversity.

Preserving Natural Habitats

Preserving the natural habitats of Brazilian Wandering Spiders is paramount to their long-term survival. Protecting forests, wetlands, and other ecosystems ensures the spiders have suitable areas to thrive. Implementing sustainable land use practices and conservation policies that consider the needs of these spiders and their habitats can help safeguard their populations for future generations.

Captivity Breeding Programs

In certain cases, captivity breeding programs may be established to support the conservation of Brazilian Wandering Spiders. These programs provide controlled environments for breeding and rearing these spiders, ensuring genetic diversity and maintaining healthy populations. Collaboration between reputable institutions and regulatory bodies is crucial in developing and implementing successful breeding programs.

Community Involvement in Spider Protection

Engaging local communities in spider protection efforts fosters a sense of responsibility and promotes the overarching goal of conservation. Encouraging the participation of local residents in monitoring spider populations, reporting sightings, and supporting sustainable land use practices can enhance the effectiveness of conservation initiatives. Community involvement ensures that the conservation efforts reflect the needs and values of the people living in close proximity to these spiders.

Role of Zoos, Sanctuaries, and Research Institutions

Zoos, sanctuaries, and research institutions play a vital role in the conservation of Brazilian Wandering Spiders. These establishments provide controlled environments for the spiders, conduct research, and educate the public. By supporting scientific studies, raising awareness, and participating in breeding programs, these institutions contribute to the long-term conservation of these fascinating arachnids.

In conclusion, the Brazilian Wandering Spider, with its potent venom and unique hunting techniques, poses both a venomous threat and an ecological fascination. Understanding its behavior, venom, and appropriate medical treatments is crucial in mitigating risks associated with potential encounters. Conservation efforts, public education, and responsible reporting play essential roles in preserving the natural habitats of these spiders and dispelling myths surrounding them. By promoting a better understanding of the Brazilian Wandering Spider, we can foster a sense of coexistence and ensure the well-being of both humans and these remarkable arachnids.

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Mike Benkert

I'm a bug enthusiast and creator of BugsWorldWide, a blog sharing his 15 years of my experience caring for bugs. I've traveled the world bird watching and I'm committed to helping others with bug care. Contact me at [email protected] for assistance.

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Brazilian Wandering Spider: Care, Food, Habitat & Preventions

Mike Wallace

Have you ever heard of or do you know what a  Brazilian wandering spider is ? It is a big venomous spider from places like Central and South America, and people sometimes call it the  banana spider . Why? Well, we are about to find out!

Table of Contents

These wandering spiders are aggressive hunters who go out on the hunt at night. Their meals include both invertebrates (like insects) and vertebrates (creatures with a backbone, like small animals).

These spiders are super dangerous because their venom is like a powerful potion that can make people really sick or even worse. They usually hang out in tropical rainforests and even in cities, hiding in banana plants. 

So, let’s get more information about the world of this sneaky spider to learn the details about its looks, eating habits, where it lives, the venom it carries, and find out if it is genuinely risky. Ready to explore? Keep reading!

Brazilian Wandering Spider Description:

Scientific name and family:.

In Brazil, they are sometimes known as “ armed spiders ” (armadeiras), and they share the name “ banana spiders ” with a few other spiders. They have different names, but they are all talking about the same interesting spider!

The Brazilian wandering spider, scientifically known as  Phoneutria , Maximilian Perty kickstarted the Phoneutria genus in 1833. The name comes from the Greek word φονεύτρια , which means “murderess” and falls under the Animalia kingdom, Arthropoda phylum, and Arachnida class.

Within Arachnida, it is classified in the order Araneae, infraorder Araneomorphae, and Ctenidae family. The genus Phoneutria, described by Perty in 1833, includes the type species  Phoneutria fera .

This classification helps us understand where these spiders fit into the larger picture of living organisms.

The following 9 species are accepted by The  World Spider Catalog :

• Phoneutria bahiensis
• Phoneutria boliviensis
• Phoneutria eickstedtae
• Phoneutria fera
• Phoneutria keyserlingi
• Phoneutria nigriventer
• Phoneutria pertyi
• Phoneutria reidyi
• Phoneutria depilata

What do Brazilian Wandering Spider look like?

Size range:.

The spiders in the Phoneutria group can get pretty big in size. Their legs can stretch out to be 13 to 18 centimeters (5 to 7 inches) wide, and their bodies can have a range between 17 to 48 millimeters (a little more than half an inch to almost 2 inches) long.

The female Brazilian spiders can get pretty big, reaching up to 15 centimeters (5.9 inches) in length. On the other hand, the males are smaller, usually measuring around 7 centimeters (2.8 inches). They usually weigh up to 0.21 ounces.

They have long, slender legs, and even though some other spiders with different names might have longer legs, the Phoneutria spiders are champions when it comes to having the longest bodies and being the heaviest in their spider gang.

The spider’s body has two main parts. The first is the prosoma, kind of like its “head,” where you will find all eight legs, eyes, fangs (chelicera), and little multitasking arms (pedipalps).

The second part is the opisthosoma, holding the spinnerets for making silk, the back end opening (anal opening), “the lungs,” the heart, and the important bits for making baby spiders (reproductive organs).

So, the prosoma is like the front control center, and the opisthosoma is like the back office, handling things like silk-making and baby-making.

Brazilian spiders come in different colors, with most being hairy and shades of brown and gray. Some species may have lightly colored spots on their abdomen.

A distinctive feature of many species is the presence of bands of black and yellow or white on the underside of their two front legs.

Identification:

To identify a spider from the Phoneutria group, look for a dense brush of fine hairs on their leg parts. They might seem like other spiders, especially  Cupiennius , but here is how you can differentiate: 

• Phoneutria often have a dark line on the front of their palps and a thin black line on top of their head. 
• Check underneath, too; their legs usually have dark parts and light joints. Sometimes, the belly has black dots or is reddish. 
• Usually it has been observed that when they are upset, they do a cool defensive move like lifting their front legs high with a distinctive pattern. So, if you see a spider doing that dance, it is probably a Phoneutria!

Brazilian Wandering Spiders live all over the Americas, from Costa Rica to northern Argentina. They are like the residents of the jungle, chilling in forests east of the Andes in countries like Colombia, Venezuela, Ecuador, Peru, Bolivia, Brazil, Paraguay, and the Guianas.

Some, like P. reidyi, P. boliviensis, and P. fera, love the Amazon rainforest, while others prefer the Atlantic Forest in Argentina, Paraguay, and Brazil.

They have also made themselves at home in the Cerrado savanna. But if you head to northeastern Brazil, they are not around. These spiders have even taken trips to Chile and Uruguay.

Why are they called Banana Spiders?

These spiders are linked with bananas. Richard S. Vetter, a researcher at the University of California, found that these powerful spiders sometimes end up in North America and Europe by accident, hitching a ride in banana shipments.

But it is often a case of mistaken identity. Only a few Phoneutria species have been found in banana shipments, and sometimes, other spiders get the blame due to misidentification. 

What They Like to Eat or Hunt?

Their food includes flies, beetles, butterflies, moths, grasshoppers, locusts, and crickets. Occasionally, they might even feast on small creatures like amphibians, reptiles, or mice. All these diet or food findings tell us about how diversified eating habits these fascinating spiders have.

Mating and Lifecycle:

Like most spiders, the female spiders are bigger than the males. When the male spider wants to be friends, they do a little dance (vibrating his pedipalps and specialized sensory appendages) to signal his intentions to impress the female, but it is a cautious approach.

The behavior of the female can be choosy, and she might say no to a few before picking the right one. 

After the dance, sometimes, the females decide to attack them, or if she is interested, she can store the male’s baby-making material in a special place until she is ready to use it.

Then, she lays a bunch of eggs, up to 1,000 at a time, and keeps them safe in a silk egg sac. Sadly, after laying her eggs, the mom spider says goodbye. It is her way of making sure the new spiders are ready to explore the world on their own.

The lifespan of the banana spider (Phoneutria nigriventer) differs for males and females. Females usually live for 6 to 8 weeks after reaching maturity, while males have a shorter lifespan of 2 to 3 weeks after their last molt. 

Certain mammals, like coatis (Procyonidae, which includes raccoons) and other small insectivores, birds are potential predators of large wandering spiders.

These spiders got their name as wandering spiders because of the fact that they are not into web building. Instead, they stroll around the forest floor at night(nocturnal), searching for dinner.

Brazilian Wandering Spiders are active hunters and use both ambush tactics and direct attacks to catch their prey. During the day, they prefer cozy spots like under logs or in crevices, only emerging at night for their hunting adventures. These spiders do not build nests like other spider species.

While wandering spiders are not naturally aggressive towards humans, they won’t hesitate to bite if they feel cornered or threatened. Most bites happen when a spider accidentally gets trapped in clothing or bedding. 

Bite and Venom:

The bite of the armed spider is the most dangerous in the world as the venom it carries can be harmful to humans.

The danger is not just about how strong the venom is; it is also about factors like the spider’s likelihood to bite and how close it is to where people live.

These spiders often hide in houses, clothes, and other dark places during the day, making accidental bites more likely. 

While their fangs are adapted for small prey, some experts think they might give a “dry” bite in defense to save venom. Studies suggest that not all bites inject venom, and serious cases requiring antivenom are rare.

However, there have been confirmed cases of death, with symptoms appearing quickly, including:

• Severe pain
• Breathing difficulties
• Increased heart rate and blood pressure
• In severe cases, paralysis and death

The severity can depend on the spider’s sex, with females generally more dangerous. The spiders produce less venom in colder months, and a small amount can be potent enough to harm.

Fortunately, bites from Brazilian spiders are rare, and when they do occur, the exposure to the toxins is generally mild, as explained by Vetter.

Also Read: What is a Huntsman Spider? (Heteropodidae) – The Ultimate Guide

Banana Spider’s Facts:

Below are essential details about Brazilian wandering spiders:

• They hold the title for the world’s largest spiders , boasting leg spans reaching up to 15 centimeters (6 inches).
• Their venom packs a powerful punch, capable of inducing severe pain, paralysis, and, in extreme cases, fatal outcomes for humans.
• Despite their intimidating reputation, they are generally non-aggressive and resort to biting only when provoked.
• These spiders inhabit tropical rainforests and urban areas across Central and South America.
• In case someone has been bitten by this spider, he/she needs quick medical treatment to control the effects timely.

Treatment and Preventive Measures:

If bitten by a wandering spider or armed spiders, prompt medical attention is crucial. There is an antivenom for the spider’s venom, but its effectiveness is highest when administered within a few hours of the bite.

To prevent a bite:

• Wear protective clothing, use shoes and long pants when in areas where these spiders are found.
• Before wearing your clothes and shoes, make sure to check them to ensure no spiders are hiding.
• Maintain cleanliness and avoid leaving food or garbage exposed, as this can attract spiders.

These preventive measures are essential for minimizing the risk of encountering and getting bitten by Banana spiders.

Can Brazilian spiders kill humans?

Brazilian wandering spiders (Phoneutria nigriventer) are venomous and can potentially kill a human with a single bite. Their venom contains a potent neurotoxin that can cause severe pain, paralysis, and even death. 

Are Brazilian spiders poisonous?

Yes the venom of this spider is poisonous, that can cause death. While Brazilian wandering spiders are potentially dangerous, actual bites are relatively rare.

By adopting preventive measures and promptly seeking medical attention if bitten, the risk of serious complications can be significantly reduced.

Can you keep Brazilian spiders as pets?

It is strongly advised against keeping wandering spiders as pets due to their venomous nature and the potential risk to human safety.

Managing these spiders in captivity demands specialized knowledge and handling procedures to minimize the risk of bites.

Final Thoughts:

The Brazilian wandering spider, banana spider, or armed spider is a large and venomous arachnid found in Central and South America. While their potent venom can be harmful to humans, encounters are rare.

These nocturnal hunters have adopted various habitats, from rainforests to urban areas, and are associated with banana shipments. Understanding their appearance, behavior, and habitat is crucial for minimizing risks.

Seeking immediate medical attention after a bite is essential, as antivenom is available but most effective when administered promptly. Despite their fearsome reputation, the Brazilian spider remains a captivating and potentially dangerous species.

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Spiders Planet is the ultimate source of information about spiders! All the articles on this site will provide you with the most accurate and up-to-date information written by me and a group of writers, that is well-researched and fact-checked before it’s published.

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Holistic profiling of the venom from the Brazilian wandering spider Phoneutria nigriventer by combining high-throughput ion channel screens with venomics

Affiliations.

• 1 Institute for Molecular Bioscience, The University of Queensland, Brisbane, Australia.
• 2 Centre of Excellence for Innovations in Peptide and Protein Science, The University of Queensland, Brisbane, Australia.
• 3 Department of Neurotransmitters, Institute of Education and Research, Santa Casa, Belo Horizonte, Brazil.
• PMID: 36865382
• PMCID: PMC9972223
• DOI: 10.3389/fmolb.2023.1069764

Introduction: Spider venoms are a unique source of bioactive peptides, many of which display remarkable biological stability and neuroactivity. Phoneutria nigriventer , often referred to as the Brazilian wandering spider, banana spider or "armed" spider, is endemic to South America and amongst the most dangerous venomous spiders in the world. There are 4,000 envenomation accidents with P. nigriventer each year in Brazil, which can lead to symptoms including priapism, hypertension, blurred vision, sweating, and vomiting. In addition to its clinical relevance, P. nigriventer venom contains peptides that provide therapeutic effects in a range of disease models. Methods: In this study, we explored the neuroactivity and molecular diversity of P. nigriventer venom using fractionation-guided high-throughput cellular assays coupled to proteomics and multi-pharmacology activity to broaden the knowledge about this venom and its therapeutic potential and provide a proof-of-concept for an investigative pipeline to study spider-venom derived neuroactive peptides. We coupled proteomics with ion channel assays using a neuroblastoma cell line to identify venom compounds that modulate the activity of voltage-gated sodium and calcium channels, as well as the nicotinic acetylcholine receptor. Results: Our data revealed that P. nigriventer venom is highly complex compared to other neurotoxin-rich venoms and contains potent modulators of voltage-gated ion channels which were classified into four families of neuroactive peptides based on their activity and structures. In addition to the reported P. nigriventer neuroactive peptides, we identified at least 27 novel cysteine-rich venom peptides for which their activity and molecular target remains to be determined. Discussion: Our findings provide a platform for studying the bioactivity of known and novel neuroactive components in the venom of P. nigriventer and other spiders and suggest that our discovery pipeline can be used to identify ion channel-targeting venom peptides with potential as pharmacological tools and to drug leads.

Keywords: bioassays; high throughput screen; ion channel; peptide drug; phoneutria; proteomics; spider venom; venomics.

Copyright © 2023 Cardoso, Walker, King and Gomez.

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Understanding the Wandering Spider: Quick Essential Facts

Wandering spiders are a group of venomous arachnids found primarily in South America.

Among these, the Brazilian wandering spider is particularly known for its potent venom and unique behavior. They are often referred to as “banana spiders” due to their frequent encounters with humans in banana plantations.

As a reader, you might be interested in learning more about these fascinating creatures, including their habitat, hunting techniques, and the effects of their venom.

In this article, we will delve into the world of wandering spiders and provide you with all the essential information to satisfy your curiosity.

Scientific Classification and Naming

The wandering spider belongs to the genus Phoneutria , which is a part of the Ctenidae family.

These spiders are known for their potent venom and aggressive behavior. Here is the scientific classification of the wandering spider:

• Kingdom: Animalia
• Phylum: Arthropoda
• Subphylum: Chelicerata
• Class: Arachnida
• Order: Araneae
• Family: Ctenidae
• Genus: Phoneutria

Within the genus Phoneutria, two species are particularly noteworthy: Phoneutria fera and Phoneutria nigriventer, also known as P. nigriventer . These spiders are primarily found in South America and other tropical regions.

Phoneutria fera and P. nigriventer differ in some aspects. Let’s compare their features using a table:

Some key characteristics of the wandering spiders in the genus Phoneutria include:

• Potent venom that can be dangerous to humans
• Nocturnal hunters and are active at night
• Equipped with long, spiny legs for capturing prey
• Aggressive defenders of their territory

By understanding the scientific classification and differences between Phoneutria species, you can better appreciate the diversity and fascinating biology of these wandering spiders.

Identification and Appearance

Color and size.

The wandering spider, also known as the banana spider, has a distinctive appearance that can help you easily identify it in the wild.

They usually have a combination of hairy brown and black colors on their body. Their size can vary, but they are generally considered large spiders. Their size can range from 1 to 2 inches in body length.

When it comes to wandering spider’s leg span, these creatures can have an impressive reach. Their leg span can extend up to 5-6 inches.

Some key characteristics of a wandering spider’s legs include:

Habitat and Distribution

Wandering Spiders are known to inhabit various environments, including rainforests and tropical forests.

These spiders can adapt to different habitats based on their needs and availability of food sources. They prefer warm and humid places, as these conditions suit their growth and reproduction.

Geographical Coverage

Wandering spiders are found in Central and South America .

They live in forests from Costa Rica to Argentina, including Colombia, Venezuela, The Guianas, Ecuador, Peru, Bolivia, Brazil, Paraguay, and Northern Argentina.

They may also be present in some parts of the United States, particularly in the northern part of southern America.

However, they don’t inhabit countries like Australia. In summary, the Wandering Spider is mostly prevalent in the following areas:

• South America
• Central America
• Southern parts of the United States

Types of Wandering Spiders

Here’s a brief description of the major types of wandering spiders.

Brazilian wandering spiders

Also known as armed or banana spiders, these spiders are nocturnal and don’t make webs.

They are known to have been transported outside of South America in banana shipments.

Phoneutria nigriventer

These spiders contain neurotoxins that can cause cerebral changes and breakdown of the blood-brain barrier .

Their venom is medically significant and has been used in manufacturing drugs. Their bites may be fatal to children.

Ctenus captiosus

Also known as the Florida false wolf spider or tropical wolf spider, this species is found in the United States.

Some species of these spiders are large and scary-looking, but they’re only mildly venomous. Their venom is comparable to a bee sting.

Other types of wandering spiders include: Acantheis, Acanthoctenus, Africactenus, and Afroneutria.

Behavior and Diet

Aggression level.

Wandering spiders, as their name suggests, are known for their aggressive behavior .

While they won’t attack without provocation, if they feel threatened, they will not hesitate to defend themselves.

This is especially true during mating season.

Prey and Predators

In their natural habitat, wandering spiders primarily feed on insects and small vertebrates, such as:

• Insects like ants and moths
• Small amphibians

This diverse diet allows them to thrive in various ecosystems.

However, they are not top predators, as their natural predators include larger birds, mammals, and other spiders.

Nocturnal Activities

Wandering spiders are nocturnal creatures , which means they are active during the night.

During the day, they remain hidden in their retreats, often made from rolled-up leaves or small crevices.

At night, they leave their hiding spots to search for prey using their strong hunting skills.

Venom and Its Effects

Composition of venom.

The venom of the wandering spider is a complex mixture containing several toxic components.

Its main component is a potent neurotoxin, which can have severe effects on your nervous system 1 . Here’s a brief overview of its composition:

• Neurotoxins

Symptoms and Severity

A wandering spider’s venomous bite can cause a wide range of symptoms, depending on the severity of envenomation. These symptoms may include 2 :

• Mild to moderate pain
• Redness and swelling at the bite site
• Irregular heartbeat
• Difficulty breathing
• Blurred vision
• High blood pressure

Some severe cases may result in life-threatening complications, such as respiratory failure or even death 2 .

Medical Treatment and Antivenom

If bitten by a wandering spider, it’s crucial to seek immediate medical attention. Treatment often involves the following steps:

• Cleaning and immobilizing the affected area
• Monitoring and managing the symptoms
• Administering antivenom if it’s available and appropriate, depending on the severity of envenomation 3

Antivenom is specific to the venom of the wandering spider and can help neutralize its effects.

However, the availability of antivenom may be limited in some regions 3 .

Always remember that prevention is better than cure: learning how to identify and avoid wandering spiders is the best way to stay safe.

Reproduction and Mating

Mating ritual.

When it’s time for reproduction, the wandering spider undergoes an intriguing mating ritual.

The male spider performs a dance to attract the female by displaying his brightly colored legs and vibrating his body.

During the process, the male also produces a sperm web and transfers his sperm to the female’s reproductive organs using his pedipalps.

Egg Sacs and Offspring

After the mating process, the female wandering spider will create an egg sac to protect her eggs.

The sac consists of silk and can hold hundreds of eggs. She then attaches it to a safe hiding place, usually against a protective surface or within a secure web.

The female often guards the egg sac to ensure the protection of her offspring until they hatch.

Once the spiderlings hatch, they are known to be highly independent.

They disperse quickly and start their own journey, fending for themselves soon after emerging from the egg sac.

As they grow, they’ll go through a series of molts before reaching adulthood and beginning their own reproductive cycle.

Danger and Defense Mechanisms

The Wandering Spider is known to be one of the most dangerous spiders in the world.

Although they can potentially kill humans, fatalities are rare due to their reluctance to bite.

Oddly enough, their venom can cause an involuntary erection in men, alongside other painful symptoms.

Here are some ways the Wandering Spider protects itself and displays its dangerous nature:

• Fangs : These spiders are equipped with strong, sharp fangs that can easily pierce human skin, allowing them to inject their venom with ease.
• Venom : Their venom is potent and can cause severe pain, inflammation, and other adverse effects. In rare cases, it can even lead to death.

While interacting with Wandering Spiders, be cautious and observe them from a safe distance.

Knowing their defense mechanisms will help you respect their space and avoid any unpleasant encounters.

Remember, it’s essential to be informed and aware when dealing with these fascinating, yet dangerous creatures.

Comparison with Other Dangerous Spiders

Comparison to black widow.

The black widow spider is notorious for its potent venom, but the wandering spider has a stronger venom overall.

Both spiders are capable of causing severe symptoms, but the black widow’s venom is primarily neurotoxic, affecting your nervous system.

In contrast, the wandering spider’s venom can cause both neurotoxic and cytotoxic effects, potentially damaging your nerves and cells.

• Potent neurotoxic venom
• Red hourglass marking
• Stronger venom (neurotoxic and cytotoxic)
• No distinct marking

Comparison to Brown Recluse

The brown recluse spider is known for its necrotic venom that can lead to tissue damage and sometimes requires medical intervention.

While both the brown recluse and wandering spider can produce venomous bites, wandering spiders are considered more dangerous due to the potency of their venom and the severity of their bite symptoms.

• Necrotic venom
• Dark violin-shaped marking

Comparison to Wolf Spider

Wolf spiders are frequently mistaken for more dangerous spiders due to their size and appearance.

Although they can bite, their venom is not particularly potent and generally only causes mild itching, redness, and swelling.

In comparison, the wandering spider’s venom is far more dangerous, and its bite can result in serious symptoms, requiring immediate medical attention.

• Large and hairy
• Smoother appearance

Comparison to Sydney Funnel-Web Spider

The Sydney funnel-web spider is another highly venomous spider known for its potentially lethal bites.

While both spiders possess powerful venom, the wandering spider has a broader range of symptoms due to the combination of neurotoxic and cytotoxic effects.

In conclusion, wandering spiders are more dangerous than wolf spiders but their venom’s effects are more varied compared to black widows, brown recluses, and Sydney funnel-web spiders.

Be cautious around these spiders and seek medical help if bitten.

Interesting Facts and Guinness World Records

The Wandering Spider, also known as the Brazilian Wandering Spider, is a fascinating creature that has caught the attention of many.

They belong to the genus Phoneutria , which means “murderess” in Greek, giving you an idea of their potency. Let’s explore some interesting facts about this spider and its place in the Guinness World Records.

First, you might be curious about their venom. The Wandering Spider is known for having one of the most potent venoms among spiders.

In fact, it holds the Guinness World Record for the most venomous spider. Their venom contains a potent neurotoxin that can cause severe symptoms, including difficulty breathing, high blood pressure, and intense pain.

Apart from their venom, their behavior is also quite intriguing. These spiders are called “wandering” because they are known for actively hunting their prey rather than spinning webs to catch them.

They are mostly nocturnal creatures and, during the day, can be found hiding in logs or dark crevices.

Here are a few more notable characteristics of the Wandering Spider:

• Females are larger than males, with a body length of up to 1.6 inches (4 cm).
• They have eight eyes, arranged in two rows, which help them in hunting.
• The Wandering Spider is primarily found in Central and South America, particularly in Brazil.
• They are known to show aggression when threatened.

While the Wandering Spider is a marvel of the arachnid world, it’s essential to keep a safe distance from them due to their venomous nature.

However, their unique characteristics and record-breaking venom potency make them a fascinating subject for those interested in the natural world.

Prevention and Safety Measures

To protect yourself from wandering spiders, there are some simple safety measures you can take.

Firstly, be cautious in areas where these spiders may live, such as dark and warm spaces. For example, avoid reaching into crevices or lifting piles of wood without inspecting them first.

Always wear appropriate shoes when outdoors, particularly in wooded or grassy areas. This can help prevent bites on your feet or ankles.

Reduce the risk of wandering spiders entering your home by sealing gaps and cracks. This minimizes the chance of the spiders finding a way inside.

Regularly clean your living spaces, paying special attention to dark and hidden areas. By maintaining a clean environment, you’ll discourage wandering spiders from making themselves at home.

When out in nature, avoid disturbing spider habitats like webs or egg sacs. This can prevent agitating wandering spiders, reducing your chance of accidental encounters.

Remember, wandering spiders can be dangerous, but by taking these precautions, you can significantly reduce your risk of encountering them or being bitten. Stay safe and always be aware of your surroundings.

In summary, wandering spiders, particularly those in the genus Phoneutria, are a group of venomous arachnids predominantly found in Central, South America and parts of Southern United States.

These spiders, including the Brazilian wandering spider, are known for their potent venom, nocturnal hunting habits, and aggressive defense mechanisms.

Their venom, containing neurotoxins and other components, can cause severe symptoms in humans, making them one of the most dangerous spider species.

Despite their fearsome reputation, fatalities are rare, and they play a vital role in their ecosystems.

It’s important to respect their space and take preventive measures to avoid encounters. Understanding these spiders’ behavior, habitat, and characteristics can help in appreciating their role in nature while ensuring safety.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2857337/ ↩

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3851068/ ↩ ↩ 2

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6560916/ ↩ ↩ 2

Reader Emails

Over the years, our website, whatsthatbug.com has received hundreds of letters and some interesting images asking us about wandering spiders. Scroll down to have a look at some of them.

Letter 1 – Wandering Spider from Ecuador

Hi Michele, There is a resemblance to the Dolomedes Fishing Spiders, and finding it near a river lends credence to that possibility. Eric Eaton noticed this posting and has this to say: ” Ok, the spiders from Ecuador and Costa Rica: They are most likely NOT wolf spiders, but wandering spiders, either in the family Ctenidae or Sparassidae. They tend to be more common, and even larger than, wolf spiders in the tropics. At least one species, Phoneutria fera, is extremely aggressive, with potentially deadly venom. Do not mess with large spiders in Central and South America! The venomous types are very difficult to distinguish from harmless species, and in any event, a bite is going to be really painful. These spiders sometimes stow away in bananas, houseplants, and other exported goods, so they can show up in odd places. Be careful where you put your hands.”

Update:  May 14, 2013 We now have a confirmation that this is a Wandering Spider, Phoneutria fera , and it is a dangerous species.  See Encyclopedia Britannica and Animal Corner .

Letter 2 – Brazilian Wandering Spider: Most Venomous Animal

Hi Martin, We are happy you were able to write to us after your encounter with this Brazilian Wandering Spider and are thrilled to be able to post your story and photos to our site. We started to research, and our first hit has a different species name. Phoneutria fera is described as: “The Brazilian Wandering Spider is not for the ‘pet keeper’. Brazilian Wandering Spiders are extremely fast, extremely venomous, and extremely aggressive. These large and dangerous true spiders are ranked among the most venomous spiders known to man. In fact, the Brazilian Wandering Spider is the most venomous spider in the New World! In South America, these true spiders are commonly encountered in peoples’ homes, supposedly hiding in peoples’ shoes, hats, and other clothes. The Brazilian Wandering Spider does not remain on a web, rather, it wanders the forest floor, hence the name.” Our favorite information on Wikipedia is that Phoneutria is Greek for “murderess”. Here is one final tidbit about the effect of the bite of the Brazilian Wandering Spider on the human male .

Letter 3 – Possibly Wandering Spider from Ecuador

Dear Mike, This is really an interesting Spider, but other than to say it appears to be a hunting spider that does not build a web to entrap prey, we aren’t sure about its identity.  Many hunting spiders can jump quite well.  It looks very much like the spider in a posting in our archives, also from Ecuador, that we identified as possibly a Wandering Spider in the genus Phoneutria, a venomous and potentially dangerous genus .  The spotted legs on your individual look like the spotted legs on an individual in an image on Wikipedia of a Wandering Spider in the genus Phoneutria .  There are many images of Brazilian Wandering Spiders on Primal Shutter and we believe that might be a correct identification for your individual.

Thank you for the information.  After reading more about the spider, I’m glad it didn’t jump! Mike

Letter 4 – Possibly Wandering Spider from Ecuador

Dear Carl, We believe, though we are not certain, that this might be a Wandering Spider in the genus Phoneutria, and you may read more about Wandering Spiders on the Museum für Naturkunde Karlsruhe website where it states:  “There is no doubt that the venom of some of the species is quite potent for mammals, including humans.”  We eagerly welcome additional opinions on this identification.  Perhaps Cesar Crash of Insetologia can provide something.  In the future, please submit a single species per submission form as it makes it extremely difficult for us to categorize postings with multiple species.

Letter 5 – Wandering Spider from Belize

Hi Karl, Thanks for allowing us to post your excellent image of a Wandering Spider, Cupiennius salei .  The species is pictured on iNaturalist .

Bugman aka Daniel Marlos has been identifying bugs since 1999. whatsthatbug.com is his passion project and it has helped millions of readers identify the bug that has been bugging them for over two decades. You can reach out to him through our Contact Page .

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Piyushi is a nature lover, blogger and traveler at heart. She lives in beautiful Canada with her family. Piyushi is an animal lover and loves to write about all creatures.

8 thoughts on “Understanding the Wandering Spider: Quick Essential Facts”

Hi Michele, I am an Ecuadorian scientist and specialized on spiders, I would like to find one like yours, I can say that, almost without doubt, you found the Phoneutria itself, it is the Phoneutria fera, look at this picture: http://www.google.com/imgres?imgurl=http://4.bp.blogspot.com/-bFH9qzT0F7U/T_2sZuk6xAI/AAAAAAAAAGY/8jnMVcPOcNI/s1600/phoneutria_fera2.jpg&imgrefurl=http://rangerbaiano.blogspot.com/2012/07/animais-peconhentos-e-venenosos.html&usg=__iCWEz7S86xub6RAyvXTER6HBaco=&h=864&w=834&sz=215&hl=es-419&start=6&zoom=1&tbnid=jjOROVO9h-vKXM:&tbnh=145&tbnw=140&ei=99eRUY6xKo2K9QTLvYCoDQ&prev=/search%3Fq%3Dphoneutria%2Bfera%26sa%3DN%26hl%3Des-US%26sout%3D1%26tbm%3Disch%26prmd%3Divns&itbs=1&sa=X&ved=0CDYQrQMwBQ Can you see the similarities?, unfortunately the spider might be in a better life today 🙂 Another thing, when you want identifications you should take a picture in front, the under part, and the upper part, as well as some characteristics about behaviour like how they react when you approach. The Phoneutria is a very agressive one.. best wishes, bye.

Hi Miguel, Thanks so much for the comment. This is a seven year old posting and we did not have the ability to post comments when it was originally posted online. We have made an update on What’s That Bug? and your comment is greatly appreciated.

Ah, there is also needed the size and the picture of its face so we can see the eye arrangement, depending on that it could also be pisauridae, but I stay in Ctenidae..

This is a female Cupiennius sp. wandering spider.

Perhaps surprisingly, this ubiquitous large spider of the Mindo area appears to be undescribed to species level.

Although one is indeed best advised to exercise caution in the presence of large ctenids, members of the genus Cupiennius are not known to be dangerously venomous (Barth, 2002). By way of confirmation, my girlfriend, Shannon Bowley, managed to be bitten by a mature female of this Mindo species in 2013 – she felt only mild effects, equivalent to a bee sting.

Thanks for this valuable information.

I’m planning a trip to Ecuador and I’m fearing these spiders. Do they get in houses? Any tips to keep them out, so I can sleep at night?

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The World’s Most Venomous Spider: The Brazilian Wandering Spider

Published: August 10, 2023

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Few things fear people’s hearts, like the mention of venomous spiders. According to the Guinness World Records , venomous spiders are some of the most dangerous creatures on Earth, and the Brazilian Wandering Spider is the world’s most venomous spider. This elusive arachnid is found in the rainforests of South America, more specifically in Brazil, Argentina, and French Guiana. ‘

It is considered one of Brazil’s most significant public health threats, with a potent venom capable of causing extreme pain, paralysis, and even death. Despite its fearsome reputation, however, the Brazilian Wandering Spider is a fascinating and unique creature with many incredible features that make it stand out from other spiders.

Jump ahead to any section below!

Brazilian Wandering Spider’s Habitat And Range

The Brazilian Wandering Spider is found in the rainforests of South America. These rainforests are the world’s largest and most biodiverse terrestrial ecosystems, covering an area of approximately 6.7 million square kilometers. This equates to roughly 40% of the total area of South America and spans nine countries, including Brazil, Peru, and Colombia.

The Amazon rainforest, the most extensive rainforest on Earth, plays an essential role in the Brazilian Wandering Spider’s habitat and range. The spider relies heavily on the dense canopy of trees in these rainforests for shelter and protection. The Amazon rainforest also provides the spider with an abundant prey source, including insects, small mammals, and other spiders.

The South American rainforests are characterized by their warm and humid climate, which creates the ideal conditions for the Brazilian Wandering Spider to thrive. These ecosystems also have a high rainfall throughout the year, which sustains the dense vegetation and creates an ideal environment for spiders to hunt and reproduce.

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Specific Locations The Brazilian Wandering Spider Is found

The Brazilian Wandering Spider is most commonly found in Brazil. It can also be found in other parts of South America, such as Argentina and French Guiana. In Brazil, the spider can be found in various regions. This includes the Amazon rainforest, the Atlantic forest, and the Caatinga ecosystem.

The Atlantic forest, a tropical forest that spans the Brazilian coast, is also an important habitat for the Brazilian Wandering Spider. This ecosystem is under threat due to deforestation and urbanization. This has resulted in the loss of the spider’s natural habitat.

In French Guiana, the spider is present in the Guiana Amazonian Park, a protected area of the Amazon rainforest managed by the French government. The Guiana Amazonian Park is home to an array of biodiversity, including monkeys, jaguars, and over 400 species of birds. This makes it an important region for the Brazilian Wandering Spider’s survival.

Brazilian Wandering Spider’s Characteristics

It is a remarkable arachnid species found in the rainforests of South America. It has numerous unique characteristics from other spiders. The Spider is large and intimidating, measuring up to 10 inches (25 cm) in leg span. Its body size ranges from 0.98 to 1.77 inches (2.5 to 4.5 cm) in length. This makes it one of the biggest spider species in the world. It has eight long and hairy legs, which help it move quickly and climb trees effortlessly.

The spider is striking, with a shiny brown or black body covered in short fine hairs. Its legs are also covered in fine hairs with dark stripes or spots. These markings have a unique pattern that helps to identify the spider from other species. Unlike most spiders, the Brazilian Wandering Spider doesn’t have a distinct web-spinning organ. But instead moves around the forest floor and trees in search of its prey.

Behavioral Patterns

They are aptly named because they are known to wander about and can cover a great distance while searching for food and mates. It’s primarily active at night and is a solitary hunter that preys on insects, spiders, rodents, and even small snakes. It has a unique hunting technique where it grabs its prey with its front legs and sinks fangs into it, injecting a potent venom that can quickly immobilize and kill its victim. Interestingly, this spider can adjust the amount of venom it injects to fit the size and the species of its prey, a skill unique to this spider species.

During the mating season, the Brazilian Wandering Spider’s behavior changes drastically. The males will approach females cautiously, tapping on the female’s web to signal their interest. They then perform a complex courtship display that involves waving their front legs, drumming their pedipalps, and vibrating on webs. The more intricate the display, the higher the chances of mating with the female.

Brazilian Wandering Spider’s Venom

The venom of the Brazilian Wandering Spider is considered one of the deadliest in the world. The potency of the venom is due to the presence of a potent neurotoxin called PhTx3. This toxin is responsible for the spider’s ability to paralyze its prey and defend itself from predators.

In scientific studies, the venom of the Brazilian Wandering Spider has proven to be significantly more toxic than other venomous spiders, such as the Black Widow and the Brown Recluse. The effectiveness of this venom is a result of its unique chemical structure, which allows it to attack the nervous system of its victim and cause significant damage.

Check out: Bengal Tiger Vs. Western Diamondback Rattlesnake .

Effects Of Venom On Humans And Animals

The effects of the venom of the Brazilian Wandering Spider can be devastating. When bitten, victims may experience severe pain, muscle spasms, and even paralysis. In extreme cases, the venom can cause respiratory failure, resulting in death.

While the venom initially causes pain and discomfort, it can lead to severe, long-lasting symptoms. In addition to causing physical harm, the venom can also have psychological effects, leading to anxiety, panic attacks, and even PTSD.

Animals are also susceptible to the venom of the Brazilian Wandering Spider . Small animals, such as mice and birds, are often preyed upon by the spider, while larger animals, such as dogs and cats, can be bitten accidentally.

Public Health Threat And Prevention

The Brazilian Wandering Spider is a significant public health threat in Brazil, with hundreds of bites reported yearly. The spider has a wide distribution throughout the country, and its venom is particularly toxic, making it a serious risk to human health.

Also, the spider is especially dangerous because it is highly aggressive and can be found in homes, gardens, and public spaces. This means that anyone can encounter the spider, regardless of location or occupation, making it difficult to prevent bites.

Measures Taken To Prevent Bites And Treat Victims

To prevent bites from the Brazilian Wandering Spider, it is essential to take steps to reduce the risk of encountering the spider. This may include keeping homes and gardens free of clutter and debris, sealing windows and doors, and avoiding areas where the spider is known to live.

In the case of a bite, it is important to seek medical attention immediately. While no anti-venom is available, medical professionals can offer supportive care to alleviate symptoms and prevent complications.

Frequently Asked Questions

The Brazilian Wandering Spider’s venom contains a potent neurotoxin called PhTx3, which can cause extreme pain, muscle spasms, and paralysis in humans. This venom is 20 times more deadly than a rattlesnake’s and can cause respiratory failure in victims.

It is primarily found in South and Central America, especially in the Amazon rainforest. They hide in dark, moist environments such as piles of leaves, tree trunks, and burrows.

To avoid contact with the Brazilian Wandering Spider, it is important to inspect your surroundings, especially before putting on shoes or reaching into piles of debris. It is advisable to wear gloves and long sleeves when working outdoors or in areas where these spiders may be present. If you suspect a Brazilian Wandering Spider has bitten you, seek medical attention immediately.

Overall, the Brazilian Wandering Spider is a fascinating and deadly spider that deserves respect and caution. Though its venom is incredibly potent, the spider itself is a remarkable creature. Its ability to move quickly and seamlessly across the ground makes it a formidable predator in its native habitats. 

Despite its fearsome reputation, we must learn to coexist with this remarkable arachnid, preserving its unique place in the natural world. Given the immense diversity and beauty of our planet’s biodiversity, the Brazilian Wandering Spider reminds us that we still have much to learn about the remarkable creatures that share our planet.

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Brazilian Wandering Spider Facts: What Happens If It Bites You?

Brazilian wandering spiders belong to the genus Phoneutria  and are represented by eight spider species that are native to Central America and South America. This spider group is also collectively known as armed spiders and banana spiders. In Brazil, they are locally known as "aranha armadeira" which means armed spider. With these, some people often wonder if a person can survive after being by a member or species of this group.

What is a Brazilian Wandering Spider?

The genus Phoneutria , which the Brazilian wandering spider  and related spider species belong to, was first described in 1833 when two species was included on it. The following century saw various scientists to move the Phoneutria species between genera Phoneutria  and Ctenus . In 1936, Mello-Leitao restored Phoneutria  and currently contains eight species, as reported by the University of Florida.

Banana spiders, a name given to the arachnid group due to their frequent presence on banana leaves, are large and robust arachnids in the family Ctenidae .   They resemble the morphological appearance of wolf spiders. In addition, their body length can grow ranging from 17 and 48 millimeters and their leg span can reach 180 millimeters. Their distinct color ranges from light brown, brown, or grey. Furthermore, the natural habitats of armed spiders are forests.

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What Happens If It Bites You?

The natural prey of Brazilian wandering spiders includes small animals like crickets, mantids, and katydids, and larger ones like bats, frogs, and lizards. However, they can still bite humans and other animals not native in their habitats. In the past, scientists have identified that the bite of banana spiders living in Central and South American rain forests can lead to shortness of breath and excessive salivation.

According to wildlife experts, a Brazilian wandering spider bite can also lead to other serious symptoms, including increased blood pressure, above-normal pulse, and unusual respiratory rate, as well as extreme pain, hours-long penile erection, and death, in some cases. These spiders inject neurotoxin venom to its bitten victim and can be deadly to humans, particularly for children. However, it is not the world's deadliest spider.

Venomous Spider

In a study published in the journal Frontiers  in February 2023, researchers stated that the Brazilian wandering spider is amongst the world's most dangerous venomous spiders in the world. In Brazil, there have been an estimated 4,000 envenomation accidents of Phoneutria nigriventer  spider species each year in Brazil. Additional symptoms were also observed, including blurred vision, priapism, and vomiting.

The armed spider only follows the world's most venomous spider, which according to the Guinness World Records , is the Sydney funnel-web spider ( Atrax robustus ).

Like the Brazilian wandering spider, the venom of A. robustus  can be neutralized by anti-venoms but some cases still lead to deaths when these arachnids bite a human, who did not receive any medical attention. Experts also weigh that the mortality of venom depends on the amount that enters a human body.

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Original research article, holistic profiling of the venom from the brazilian wandering spider phoneutria nigriventer by combining high-throughput ion channel screens with venomics.

• 1 Institute for Molecular Bioscience, The University of Queensland, Brisbane, Australia
• 2 Centre of Excellence for Innovations in Peptide and Protein Science, The University of Queensland, Brisbane, Australia
• 3 Department of Neurotransmitters, Institute of Education and Research, Santa Casa, Belo Horizonte, Brazil

Introduction: Spider venoms are a unique source of bioactive peptides, many of which display remarkable biological stability and neuroactivity. Phoneutria nigriventer , often referred to as the Brazilian wandering spider, banana spider or “armed” spider, is endemic to South America and amongst the most dangerous venomous spiders in the world. There are 4,000 envenomation accidents with P. nigriventer each year in Brazil, which can lead to symptoms including priapism, hypertension, blurred vision, sweating, and vomiting. In addition to its clinical relevance, P. nigriventer venom contains peptides that provide therapeutic effects in a range of disease models.

Methods: In this study, we explored the neuroactivity and molecular diversity of P. nigriventer venom using fractionation-guided high-throughput cellular assays coupled to proteomics and multi-pharmacology activity to broaden the knowledge about this venom and its therapeutic potential and provide a proof-of-concept for an investigative pipeline to study spider-venom derived neuroactive peptides. We coupled proteomics with ion channel assays using a neuroblastoma cell line to identify venom compounds that modulate the activity of voltage-gated sodium and calcium channels, as well as the nicotinic acetylcholine receptor.

Results: Our data revealed that P. nigriventer venom is highly complex compared to other neurotoxin-rich venoms and contains potent modulators of voltage-gated ion channels which were classified into four families of neuroactive peptides based on their activity and structures. In addition to the reported P. nigriventer neuroactive peptides, we identified at least 27 novel cysteine-rich venom peptides for which their activity and molecular target remains to be determined.

Discussion: Our findings provide a platform for studying the bioactivity of known and novel neuroactive components in the venom of P. nigriventer and other spiders and suggest that our discovery pipeline can be used to identify ion channel-targeting venom peptides with potential as pharmacological tools and to drug leads.

Introduction

Venomous animals are a highly adapted group of organisms whose evolutionary success excelled with the emergence of venom. Spider venoms, in particular, are rich in peptide knottins specialized in modulating, often with high potency and selectivity, voltage-gated ion channels that regulate the physiology of neuronal, muscular and cardiac systems ( Cardoso and Lewis, 2018 ; Cardoso, 2020 ). Although such effects can be deleterious to envenomated animals, venom components can be tailored to selectively modulate ion channels in pathways of complex diseases such as chronic pain, motor neuron disease, and epilepsy. This has been demonstrated for numerous spider venoms ( Smith et al., 2015 ; Cardoso and Lewis, 2018 , 2019 ), including the venom of the infamous South American ctenid spider Phoneutria nigriventer , often referred as Brazilian wandering spider, banana spider or “armed” spider ( Peigneur et al., 2018 ). Besides its clinical relevance due to frequent envenomation cases in Brazil, with approximately 4,000 cases per year ( Isbister and Fan, 2011 ; Gewehr et al., 2013 ), P. nigriventer venom contains peptides that have therapeutic effects in a range of disease models including chronic pain ( Pedron et al., 2021 ; Cavalli et al., 2022 ), Huntington’s disease ( Joviano-Santos et al., 2022 ), glaucoma ( da Silva et al., 2020 ) and erectile dysfunction ( Nunes da Silva et al., 2019 ).

Initial studies of P. nigriventer venom employed fractionation via gel filtration and reversed-phase chromatography to separate the venom into five distinct groups of peptides based on their molecular weight and hydrophobicity properties; these groups were named PhTx1 to PhTx5 ( Peigneur et al., 2018 ). PhTx1–4 comprise cysteine-rich peptides that are active on voltage-gated calcium (Ca V ), sodium (Na V ) and potassium (K V ) channels, while PhTx5 is comprised of short linear peptides, with a total of 34 peptides identified ( Peigneur et al., 2018 ). Proteotranscriptomic studies of P. nigriventer venom revealed additional peptides with high similarity to those previously described, but very few have been characterised pharmacologically ( Cardoso et al., 2003 ; Richardson et al., 2006 ). This represents an obstacle to the exploration of the therapeutic potential of P. nigriventer venom.

Advances in venom-peptide research have yielded high-throughput cellular screens for the discovery and pharmacological characterisation of naturally occurring molecules with activity at ion channels and receptors in physiological pathways ( Cardoso et al., 2015 ; Cardoso et al., 2021 ). These methods require only a small amount of venom compared to more traditional methods and allow the identification of therapeutically relevant peptides in the early stages of the screening. Besides drug development applications, these same bioassays can assist in unravelling the bioactivity of crude and fractionated venoms from biomedically relevant venomous animals to support studies of evolution and antivenom development, but much work remains to be done in this field.

This study aimed to provide a proof-of-concept in applying high-throughput cellular screens for multiple neuronal ion channels along with proteomic studies of fractionated venom to rapidly characterise spider venoms in terms of bioactive components. It was anticipated that such a pipeline would support envenomation and evolutionary studies and the development of therapeutics from animal venoms. The venom of P. nigriventer was selected as a model system due to its medical relevance, the considerable number of therapeutically relevant peptides already uncovered in the venom, and the wide knowledge base available. Our approach enabled identification of potent modulators of voltage-gated ion channels which were classified into four families of neuroactive peptides based on their activity and structures. In addition to the previously characterised neuroactive peptides in the P. nigriventer venom, we identified 27 additional cysteine-rich venom peptides in which neuroactivities are underexplored. This work contributes to the on-going discovery and structure-function characterisation of spider-venom peptides. Moreover, our bioassay pipeline can be used to guide future research into the discovery of venom peptides that modulate the activity of ion channels, and their development as pharmacological tools and drug leads.

Materials and methods

We applied a holistic approach combining methods in high throughput screens for ion channels, venom proteome, venom gland transcriptome and modelling of peptides as described in Figure 1 .

FIGURE 1 . Flowchart of the venom peptide discovery pipeline applied in this study. Expanding from the traditional assay-guided fractionation, we applied HTS bioassays to characterize the pharmacology of venom peptides on multiple ion channels, followed by the identification of peptide masses and primary sequences using proteome and transcriptome. Ultimately, the three-dimensional structure of venom peptides was determined using in silico molecular modelling.

Cell culture

The human neuroblastoma cell line SH-SY5Y was maintained at 37 ° C in a humidified 5% CO 2 incubator in Roswell Park Memorial Institute (RPMI) medium supplemented with 15% foetal bovine serum (FBS) and 2 mM L-glutamine. Replicating cells were sub-cultured every 3–4 days in a 1:5 ratio using 0.25% trypsin/EDTA.

Venom fractionation

Crude venom milked from male and female specimens of P. nigriventer was kindly provided by Prof. Marcus Vinicius Gomez from the Institute of Teaching and Research of Santa Casa de Belo Horizonte, Belo Horizonte, Brazil. Venom (lyophilised, 1 mg) was dissolved in 100 μL Milli-Q water containing 0.05% trifluoroacetic acid (TFA) (Auspep, VIC, AU) and 5% acetonitrile (ACN) and centrifuged at 5,000 × g for 10 min to remove particulates. Venom was fractionated by reversed-phase high performance liquid chromatography (RP-HPLC) using a C18 column (Vydac 4.6 mm × 250 mm, 5 μm, Grace Discovery Sciences, United States) with a gradient of solvent B (90% ACN in 0.045% TFA) in solvent A (0.05% TFA). The gradient was 5% B for 5 min, followed by 20%–40% solvent B over 60 min at a flow rate 0.7 mL min −1 . Peaks were collected every minute, with fraction 1 eluted between 1 and 2 min and so on for the other fractions. Venom fractions were lyophilised before storage at –20°C.

Calcium influx assays

Venom fractions were screened for neuroactivity at human (h) Na V , Ca V 1, Ca V 2 and the α7 subtype of the human nicotinic acetylcholine receptor (nAChR-α7) as previously described ( Cardoso et al., 2015 ). Briefly, SH-SY5Y cells were plated at 40,000 cells per well in 384-well flat clear-bottom black plates (Corning, NY, United States) and cultured at 37 ° C in a humidified 5% CO 2 incubator for 48 h. Cells were loaded with 20 μL per well Calcium 4 dye (Molecular Devices) reconstituted in assay buffer containing (in mM) 140 NaCl, 11.5 glucose, 5.9 KCl, 1.4 MgCl 2 , 1.2 NaH 2 PO 4 , 5 NaHCO 3 , 1.8 CaCl 2 and 10 HEPES pH 7.4 and incubated for 30 min at 37 ° C in a humidified 5% CO 2 incubator. For the hCa V 1 assay, the dye was supplemented with 1 μM ω-conotoxin-CVIF (CVIF) to inhibit Ca V 2, and in the hCav2 assay the dye was supplemented with 10 μM nifedipine to inhibit Ca V 1. For the nAChR-α7 assay, the dye was supplemented with PNU-120596 (Sigma-Aldrich), a positive allosteric modulator of nAChR-α7. Venom fractions were assayed in singleton for each ion channel tested. Fluorescence responses were recorded using excitation at 470–495 nm and emission at 515–575 nm for 10 s to set the baseline, then 300 s after addition of 10% venom fraction serial diluted at 1, 1:10, and 1:100, and for a further 300 s after addition of 50 μM veratridine for hNa V , 90 mM KCl and 5 mM CaCl 2 for hCa V, and 30 μM choline for nAChR-α7.

Venom fractions eluting between 10 and 45 min on RP-HPLC were analysed by mass spectrometry to investigate the masses and primary structures of their peptide components. Native mass determinations were carried out with 20% of each fraction dried by vacuum centrifuge and resuspended in 20 μL 1% formic acid (FA), followed by analysis using by liquid chromatography/tandem mass spectrometry (LC-MS/MS). For identification of primary structures, 20% of each peptide fraction was reduced and alkylated by adding 40 μL of reagent composed of 4.875 mL ACN, 4.5 mL ultrapure water, 0.5 mL 1M ammonium carbonate pH 11.0, 100 μL 2-iodoethanol and 25 μL triethylphosphine, and incubating for 1 h at 37°C. Samples were speed dried in a vacuum centrifuge, and digested with 40 ng/μL trypsin in 50 mM ammonium bicarbonate pH 8.0 and 10% ACN overnight at room temperature. Trypsin was inactivated by adding 50 μL solution containing 50% acetonitrile and 5% formic acid (FA), dried in speed vacuum centrifuge, and resuspended in 1% formic acid.

LC-MS/MS samples were loaded onto a 150 mm × 0.1 mm Zorbax 300SB-C18 column (Agilent, Santa Clara, CA, United States) on a Shimadzu Nano LC system with the outflow coupled to a SCIEX 5600 Triple TOF (Framingham, MA, United States) mass spectrometer equipped with a Turbo V ion source. Peptides were eluted using a 30 min gradient of 1%–40% solvent B (90% ACN/0.1% FA) in solvent A (0.1% FA) at a flow rate of 0.2 mL/min. For MS1 scans, m/z was set between 350 and 2,200. Precursor ions with m/z 350–1,500, charge of +2 to +5, and signals with >100 counts/s (excluding isotopes within 2 Da) were selected for fragmentation, and MS2 scans were collected over a range of 80–1,500 m /z . Scans were obtained with an accumulation time of 250 ms and a cycle of 4 s.

A database of possible peptide sequences produced in P. nigriventer venom glands was compiled using a published venom-gland transcriptome ( Diniz et al., 2018 ), from which open reading frames (ORFs) longer than 30 amino acids were identified and translated by TransDecoder. A list of 200 common MS contaminants was added to the translated ORFs, which was used as a sequence database to compare to mass spectral data using the Paragon algorithm in Protein Pilot 2.2 software (AB SCIEX). We report only peptides for which more than two tryptic fragments were detected with >95% confidence, or where one tryptic fragment was detected, and a secretion signal peptide was predicted by SignalP5.0.

Molecular modelling

Venom peptides identified in this study were selected based on their cysteine-rich scaffold and bioactivity, and their three-dimensional (3D) structure were predicted using the AlphaFold 2 algorithm ( Jumper et al., 2021 ). All 3D structures displayed were from unrelaxed models ranked 1 for each peptide prediction. 3D structures were visualised and analysed using PyMol ( Pymol, 2023 ).

Data analysis

Fluorescence traces from singletons were evaluated using the Maximum-Minimum or Area Under the Curve values generated after addition of ion channel activator. Data were normalised against the negative control (PSS buffer control) and positive control (ion channel activator) for each assay and corrected using the response over baseline from 1 to 5 s. No statistical analyses were required in this study.

Screening of P. nigriventer venom fractions

Fractionation of 1 mg of P. nigriventer ( Figure 2A ) crude venom using RP-HPLC produced numerous peaks eluting between 20% and 40% solvent B, and fractions eluting between 11 and 45 min were selected for pharmacological analysis ( Figure 2B ). Screening using the SH-SY5Y neuroblastoma cell line revealed strong modulation of voltage-gated ion channels including both inhibition or enhancement of ion channel activity ( Figure 2C ). Venom fractions eluting between 18 and 34 min showed strong inhibition of Ca V and Na V activity, while fractions eluting between 41 and 45 min strongly activated Ca V 2 channels ( Figure 2C , top panel). At a dilution of 1:10, these inhibitory effects persisted for both Na V and Ca V 2 channels for fractions eluting at 19–20 min and 26–34 min and was absent for Ca V 1 channels ( Figure 2C , middle panel). Fractions eluting from 21 to 25 min showed a clear preference for inhibiting only Ca V 2 channels ( Figure 2C ). Interestingly, at 1:10 dilution, channel activity enhancement was stronger on Na V channels compared to Ca V 2 channels, suggesting potential concentration-dependent synergistic effects of venom peptides modulating both Na V and Ca V 2 channels. At the highest venom dilution of 1:100, persistent inhibition of Na V channel was observed for fraction 20 (F20), while the remaining inhibitory fractions preferentially inhibited only Ca V 2 channels ( Figure 2C , bottom panel). Channel enhancement persisted for Na V channels in fractions eluting from 41 to 45 min. No potent activity was observed against nAChR-α7 at any venom concentration tested. Overall, inhibitory activity was primarily observed for fractions eluting at shorter retention times (i.e., more hydrophilic compounds), while strong ion channel activation was induced by more hydrophobic peptides with longer RP-HPLC retention times.

FIGURE 2 . Fractionation and activity of P. nigriventer venom. (A) P. nigriventer specimen displaying threat posture (photo copyright Alan Henderson, www.minibeastwildlife.com.au ). (B) RP-HPLC fractionation of 1 mg P. nigriventer venom. (C) Ion channel responses calculated from the area under the curve (AUC) after addition of selective activators for fractions 10 to 45, normalized to responses in the absence of venom fractions. (D, E) Representative fluorescence traces of the intracellular calcium responses of SH-SY5Y cells evoked by KCl + CaCl 2 in the presence of venom fractions 26 and 34 for Ca V 1, fractions 19, 26 and 34 for Ca V 2, and fractions 41–45 for both Ca V 1 and Ca V 2 channels. (F) Representative fluorescence traces of the intracellular calcium responses of SH-SY5Y cells evoked by veratridine and in the presence of venom fractions 19, 26 and 34 and fractions 41–45. (G) Representative fluorescence traces of the intracellular calcium responses of SH-SY5Y cells evoked by choline and in the presence of venom fractions 16 and 40 and fractions 41–45. Grey dotted line indicates the KCl + CaCl 2 , veratridine or choline addition.

Fluorescent traces measured upon addition of venom fractions revealed an increase in intracellular calcium ([Ca 2+ ] i ), suggesting that these venom peptides can activate closed channels as well as enhance the responses of these channels opened using pharmacological intervention ( Figures 2D–G ). This was observed for Ca V responses in the presence of 1 μM CVIF (Ca V 2 inhibitor, Figure 2D ) and 10 μM nifedipine (Ca V 1 inhibitor, Figure 2E ). In the absence of Ca V inhibitors, these [Ca 2+ ] i responses resemble the levels of Ca V 1 responses in Figure 2D as observed for F40–F45 applied in the Na V channels assay ( Figure 2F ). The activities of inhibitory fractions were mostly free from initial [Ca 2+ ] i responses upon venom addition, except for weak inhibitors observed in F19 for Na V and F40 for nAChR-α7 ( Figures 2F, G ).

Identification of peptides in P. nigriventer venom fractions

The venom of P. nigriventer has been extensively characterised in terms of composition and bioactivity ( Diniz et al., 2018 ; Peigneur et al., 2018 ), including neuronal ion channel activity and proteomics, but not by using a combined approach. In this study, by combining these approaches, we were able to rapidly identify 58 peptides and proteins in the venom. Due to the complexity of previous nomenclature for P. nigriventer venom peptides, we refer to them here using both the rational nomenclature developed for spider toxins ( King et al., 2008 ) and an identifying number (e.g., PN367) that is linked to a sequence and a list of previously used names in Supplementary Table S1 . Of the 58 identified amino acid sequences, only eight (15%) are peptides that have had their bioactivity reported in previous studies ( Figure 3A , Supplementary Table S1 ) ( Peigneur et al., 2018 ). These included the known neuroactive components μ-CNTX-Pn1a (Tx1) ( Diniz et al., 2006 ; Martin-Moutot et al., 2006 ), κ-CNTX-Pn1a (Tx3-1, PhK V ) ( Kushmerick et al., 1999 ; Almeida et al., 2011 ), ω-CNTX-Pn1a (Tx3-2) ( Cordeiro Mdo et al., 1993 ), Γ-CNTX-Pn1a [Tx4(5-5)] ( Paiva et al., 2016 ), δ-CNTX-Pn1a [Tx4(6-1)] ( de Lima et al., 2002 ; Emerich et al., 2016 ), δ-CNTX-Pn2c (Tx2-5a) ( Yonamine et al., 2004 ), ω-CNTX-Pn4a (Tx3-6) ( Cardoso et al., 2003 ; Vieira et al., 2005 ) and ω-CNTX Pn3a (Tx3-4) ( Dos Santos et al., 2002 ) ( Figure 3B ). Even among these eight peptides, only a few venom peptides have had their molecular pharmacology characterized in detail ( Peigneur et al., 2018 ), or their activities confirmed using recombinant peptides ( Diniz et al., 2006 ; Paiva et al., 2016 ; Garcia Mendes et al., 2021 ).

FIGURE 3 . Estimated levels of peptide/protein venom components identified in fractions F17 to F45, and their respective bioactivity at Na V and Ca V channels and the nAChR-α7. (A) Proportion of known and unknown venom peptides and other venom components detected in this study. (B) Venom peptides with previously reported bioactivity detected in fractions by mass spectrometry and compared to fraction bioactivity at Na V and Ca V channels and the nAChR-α7. (C) Venom peptides detected in fractions classified according to their cysteine framework I to IX ( Diniz et al., 2018 ), and compared to fraction bioactivity at Na V and Ca V channels and the nAChR-α7.

Most of the identified sequences in this study (74%) represent peptides with unexplored bioactivity; 38 (65%) of the 43 peptides identified have cysteine-rich scaffolds typical of spider-venom peptides ( Figure 3C ). Some of these venom peptides, such as PN367 and PN363, have a type I scaffold ( Diniz et al., 2018 ) and are predicted by Alphafold 2 to fold into cystine-knot scaffolds typical of spider-venom peptides ( King and Hardy, 2013 ) ( Figure 4 ). Scaffolds II-VIII either form elaborated cystine-knot folds with extra disulphide bonds, or alternative structures such as for scaffolds III and IV ( Figure 4 ). Novel peptides with high identity with other toxins and not previously described in P. nigriventer venom included: PN367 displaying identity with a Agelena orientalis venom peptide; PN369 displaying identity with a Lycosa singoriensis venom peptide, and PN365 displaying scaffold III and identity with another Lycosa singoriensis venom peptide ( Supplementary Table S1 ). Additional disulphide-rich scaffolds present in P. nigriventer venom include three peptides predicted by the algorithm HMMER to form a thyroglobulin type 1 repeat domain (E < e −17 in each case), one of which has been previously reported as U24-CNTX-Pn1a; peptide PN370 which displays high identity with a peptide found in venom of the scorpion Scorpiops jendeki and is predicted by the algorithm HMMER to form into a trypsin-inhibitor-like cysteine-rich domain (E < 2e −13 ); and the peptide PN376 that is predicted by HMMER to form a fungal protease inhibitor domain (E < e −6 ) ( Supplementary Table S1 ). Additional new scaffolds identified in this study were named following the previous suggested nomenclature ( Diniz et al., 2018 ) as X (CXCC motif, 12 Cys residues: −C−C−C−C−CXCC−C−C−C−C−C−), XI (12 Cys residues: −C−C−C−CXC−CXC−C−CXC−C−C−), XII (11 Cys residues: −C−C−CXC−CXC−C−C−CXC−C) and XIII (10 Cys residues: −C−C−C−C−C−C−CXC−C−C−), and include the peptides PN376, PN372, PN373 and PN375, and PN370, respectively.

FIGURE 4 . Diversity and estimated levels of cysteine-rich scaffolds identified in highly neuroactive RP-HPLC fractions from the venom of P. nigriventer , and their predicted 3D structures. (A) Fractions 18–20 comprised high levels of scaffolds I, II and VIII represented by the 3D structures of PN367, PN105 and PN267, respectively. (B) Fractions 26 and 27 comprised high levels of scaffolds II, and IV, and an undefined scaffold represented by the 3D structures of PN321, PN350 and PN372, respectively. (C) Fraction 34 comprised high levels of scaffolds I, II, and V represented by the 3D structures of PN003, PN292 and PN028, respectively. (D) Fractions 41 and 42 comprised high levels of scaffolds IV and V represented by the 3D structures of PN381, and PN077 and PN031, respectively.

Only 9% of the identified sequences were peptides with two or fewer Cys residues ( Supplementary Table S1 ). F17 contained a peptide (PN361) matching a C-terminally amidated peptide precursor from Araneus ventricosus identified in a genomic study ( Kono et al., 2019 ). This precursor has 70% sequence identify with the prohormone-1 like precursor from the honeybee Apis mellifera (UniProt P85798) which is believed to be cleaved to form three short peptides with neuronal activity. Another short peptide, PN366 identified in F18 and F28–F30, matches a neuropeptide in the sea slug Aplysia californica (UniProt P06518). Larger proteins were also detected in some fractions; for example, F18 and F31 contained a fragment at 58% and 70% total fraction components, respectively, matching a zinc metalloprotease from the nematode Caenorhabditis elegans (UniProt 55112) which contains a peptidase family M12A domain.

Diversity of neuroactive peptides in P. nigriventer venom

The cysteine-rich scaffolds of venom peptides identified in this study were compared to the classification previously proposed for P. nigriventer venom peptides ( Diniz et al., 2018 ) ( Figures 3C , 4 ). Peptides in fractions displaying inhibitory properties corresponded to scaffolds I, II, IV, V and VIII, as well as unnamed scaffolds, while peptides in fractions with activation properties comprised mostly the scaffold V. All of these scaffolds are inhibitor cystine knot motifs, except for scaffold IV which had the highest level in F26 represented by the peptide PN350.

Neuroactive peptides with greater hydrophilicity (i.e., those with short RP-HPLC retention times) showed pharmacological properties reminiscent of known spider-derived μ-toxins (F17 and F18) and ω-toxins (i.e., inhibition of Ca V 1 and Ca V 2 channels by F19 and F20) ( Figures 2C , 5A ). Major components driving those bioactivities were the pharmacologically characterised peptides μ-CNTX-Pn1a, ω-CNTX-Pn1a and ω-CNTX-Pn3a, as well as additional peptides with unknown activity ( Figure 4A ). As the hydrophilicity of the peptides decrease (i.e., peptides with long RP-HPLC retention times), persistent Ca V 2 inhibition was observed with maximum inhibitory activity in F26 and F27, and with the additional peptide ω-CNTX-Pn4a detected in F24 ( Figures 2C , 3B , 5B ). Interestingly, venom peptides characterized as K V modulators, such as κ-CNTX-Pn1a, were detected in fractions displaying strong inhibition of calcium influx with potential μ- and ω-pharmacology (fractions 26 and 27); it was not clear if the observed bioactivity was associated to the modulation of K V channels, or to other unexplored peptides in these fractions.

FIGURE 5 . Venom peptide content of highly neuroactive RP-HPLC fractions from the venom of P. nigriventer . (A) Identification of the cysteine-rich peptides and proteins in fractions 17–20 displaying potent inhibition of neuronal Na V and Ca V 2 channels. Positively and negatively charged residues are coloured blue and orange, respectively, hydrophobic residues are green, and cysteines are highlighted in grey box. (B) Identification of the peptide and protein content of the fractions 16 and 27 displaying potent inhibition of neuronal Na V , Ca V 1 and Ca V 2 channels. (C) Identification of the peptide and protein content of the fraction 34 displaying potent inhibition of neuronal Na V , Ca V 1 and Ca V 2 channels. (D) Identification of the peptide and protein content of the fraction 34 displaying potent activation of neuronal Na V and Ca V 2 channels. Sequences labelled with a red asterisk (*) at the C-terminal are likely C-terminally amidated.

Neuroactive peptides presenting more hydrophobic structures showed properties of μ and ω-peptides, but with preference for Ca V 2 channels as observed for fraction 34 in which the peptide Γ-Pn1a is the major component, consistent with its previously observed modulation of multiple cation channels ( Paiva et al., 2016 ); and of δ-peptides as observed in fractions 41 to 45, in which major components included the peptides δ-Pn1a and δ-Pn2c ( Figures 2C–F , and Figures 5C, D ). Notably, the main components of some of the most neuroactive fractions are peptides with unexplored bioactivity, e.g., fraction 26 ( Figures 2C , 4 , 5 ).

Pharmacological groups

Our approach allowed classification of P. nigriventer venom peptides into four major groups based on their bioactivity ( Figure 6 ; Table 1 ). Group 1 is comprised of μ and ω peptides with scaffold type VIII and more hydrophilic properties as they eluted between F17 and F21. As representatives from this group, μ-CNTX-Pn1a and ω-CNTX-Pn3a have a potential “KR electrostatic trap”, a pharmacophore described in spider-venom peptides that modulate ion channels ( Hu et al., 2021 ; Wisedchaisri et al., 2021 ), in their primary and tertiary structures ( Figure 6A ). This pharmacophore is likely composed of residues R61, K67, K70, K71, R74 and R75 in μ-CNTX-Pn1a and residues K54, K56, R59, K65, K70, R71, K73 and K74 in ω-CNTX-Pn3a. Within this group, the ω-CNTX-Pn3a homologue PN319 differs at three positions, making it an interesting candidate for further characterisation.

FIGURE 6 . Pharmacological groups identified in the most active venom fractions highlighting the “KR electrostatic trap” pharmacophore common to spider toxins that modulate the activity of ion channels. (A) Group 1 is represented by μ- and ω-spider-venom peptides with large and complex type VIII scaffold. (B) Group 2 is represented by κ- and ω-spider-venom peptides with type II and VII scaffolds. (C) Group 3 is represented by γ-spider-venom peptides with type V scaffold. (D) Group 4 is represented by δ-spider-venom peptides displaying a type V scaffold. K and R residues located in the C-terminal region of these peptides and grouped on a positively charged face are highlighted in red in the sequences and in red tubes in the corresponding 3D structures. Arrows shows the cysteine-bridge connection forming the cyclic peptide structures predicted for PN028 and PN031.

TABLE 1 . Pharmacological groups identified in this study with respective pharmacological types, cysteine-rich scaffold types, and representative venom peptides described in the literature. Unexplored peptides within each group are described in Figure 5 and/or Supplementary Table S1 .

Group 2 comprises κ and ω peptides that eluted between F17 and F28, with scaffold types II and VII ( Figure 6B ). As representatives from this group, peptides κ-CNTX-Pn1a, ω-CNTX-Pn1a and ω-CNTX-Pn4a also contain a “KR trap” pharmacophore comprised of residues R20, K23, K34, K35 and K36 for ω-Pn1a; R21, K24, K35 and K36 for κ-Pn1a; and K42, R47, K48, K49, K51, K53 and K54 for ω-Pn4a. In this group, PN107 differs from κ-CNTX-Pn1a by only two residues and is an interesting peptide for further exploration.

Group 3 is comprised of more hydrophobic Γ peptides that eluted in F33–F36 and possess a type V scaffold ( Figures 3C–D , 6C ). It is represented by Γ-CNTX-Pn1a with a potential “KR trap” comprising residues K35, R41, K42 and K43. Although Γ peptides modulate N-methyl-D-aspartate (NMDA) glutamate receptors, Γ-CNTX-Pn1a has also been reported as a β-peptide that inhibits Na V channels ( Paiva et al., 2016 ), which agrees with the results from our high-throughput ion channels assays ( Figures 2C–F , 3 ). Interestingly, Γ-CNTX-Pn1a predicted 3D structure formed a cyclic structure in which the N-terminal cysteine formed a disulfide bridge with C-terminal cysteine ( Figures 4C , 6C ). These same fractions contain other ICK peptides including PN003 and PN292 with scaffold types I and II, respectively; their pharmacological targets have not been explored but they likely contribute to the strong inhibition of Ca V channels by F34 ( Figures 2 , 3 , 4 ).

Group 4 is composed of very hydrophobic δ peptides that elute in F40–F45 and possess a type V scaffold ( Figure 2B , 6D ). It is represented by δ-CNTX-Pn1a with potential “KR trap” comprising residues K43, K44, and K45 ( Figure 6D ). In this group we also identified δ-CNTX-Pn2c which differs not only in primary structure but also in the scaffold V tertiary structure by presenting a non-cyclic structure compared to the cyclic structure predicted for δ-CNTX-Pn1a connected by the N- and C-terminal cysteines ( Figures 4D , 6D ). Beyond these known peptides, this group comprised interesting unexplored peptides such as PN032 and PN023 showing δ peptide domains and differing from Γ-CNTX-Pn1a by 12 and 11 residues, respectively.

Spiders are one of the most speciose venomous taxa, with >50,000 characterised species (see World Spider Catalog, https://wsc.nmbe.ch/statistics/ ). Their venoms are rich in neuroactive peptides that target a wide range of neuronal ion channels and receptors using mechanisms distinct from those of neurotoxins from other venomous animals such as cone snails and scorpions. The exploration of venom peptides targeting ion channels and receptors provides novel opportunities for the development of pharmacological tools to understand disease mechanisms ( Cardoso and Lewis, 2018 ; Cardoso, 2020 ) as well as provision of leads for development of therapeutics ( King, 2011 ) and bioinsecticides ( Smith et al., 2013 ).

Spiders are classified in two major groups, or infraorders ( King, 2004 ): Mygalomorphae, or so-called “primitive spiders”, includes the family Theraphosidae, or tarantulas, which are the most well studied spider venoms due to the large-size and long lifespan (often >20 years) of these spiders. Araneomorphae, or “modern spiders,” comprise >90% of all extant spider species, including the family Ctenidae in which P. nigriventer resides. Notably, despite their much greater species diversity, araneomorph venoms are underexplored compared to mygalomorphs due to their smaller size and shorter lifespan (typically 1–2 years). Our data, and those of others ( Binford et al., 2009 ; Zhang et al., 2010 ; Diniz et al., 2018 ; Peigneur et al., 2018 ), showed a great diversity of both pharmacological actions and cysteine scaffolds in araneomorph venom, which may have facilitated the highly successful araneomorph radiation. Our data also suggests Araneomorphae’s venoms may be a rich source of unique venom peptides with more diverse structures and pharmacological functions and additional biotechnological and therapeutic applications to Mygalomorphae’s venoms.

The venom from P. nigriventer comprises many exceptional peptides drug leads under development for treating a range of complex neuro disorders ( Peigneur et al., 2018 ). These peptides have been evaluated in pre-clinical models and demonstrated interesting therapeutic efficacy in reverting or preventing conditions for which treatments are limited or unavailable. For example, ω-Pn2a and ω-Pn4a showed efficacy in treating painful neuropathies such as fibromyalgia and chronic post-ischemia pain, respectively ( Pedron et al., 2021 ; Cavalli et al., 2022 ), ω-Pn4a also improved motor movement and neuroprotection in Huntington’s disease ( Joviano-Santos et al., 2022 ). The engineered peptide PnPP-19 derived from the venom peptide δ-Pn2a was efficacious in treating glaucoma ( da Silva et al., 2020 ) and erectile dysfunction ( Nunes da Silva et al., 2019 ). In our study, these therapeutic peptides showed bioactivity at neuronal Na V and Ca V channels, which greatly supports our investigative platform for the discovery of venom peptides useful for the development of efficacious drugs.

Investigative pipelines in venomic studies often focus on the elucidation of venom components based on their structures but lack clear strategies to investigate venom bioactivities ( von Reumont et al., 2022 ). Investigations using fractionated venom ( Cardoso et al., 2015 ; Cardoso et al., 2017 ; Estrada-Gomez et al., 2019 ; Cardoso et al., 2021 ) provides more defined biological functions than using crude venom due to the immense pharmacological diversity of venom, which often contains venom components with opposing activity as well as components that act synergistically ( Raposo et al., 2016 ). Considering the large number of extant spiders and consequently the exceptionally large number of venom components available for investigation, high-throughput (HT) functional bioassays are essential for developing a holistic understanding of venom pharmacology, and they provide a complement to venomic studies.

A recent study by us using HT bioassays to investigate the ion channel targets of Australian funnel-web spider venoms recaptured current taxonomy and revealed potential drug targets to treat severely envenomated patients ( Cardoso et al., 2022 ). In this present study, we also demonstrated the feasibility of applying HT functional bioassays to investigate spider venom components that mediated the activity of voltage-gated ion channels. We were able to capture all known venom components and associated bioactivities using a HT functional assay as well as several new unexplored venom peptides that warrant further exploration. This was achievable only by combining HT bioassays with transcriptomic and proteomic approaches. Although this pipeline provides a robust holistic overview of spider venoms, bioactive components are present in varying concentrations in each fraction, which may affect bioactivity through synergistic effects, and overlook the activity of less abundant components.

The complexity of the cysteine-rich scaffolds in P. nigriventer venom peptides unraveled in this study suggests that further exploration utilising recombinant or synthetic peptides might be challenging but essential, and these could also benefit from modern strategies utilizing HT recombinant expression or chemical synthesis ( Pipkorn et al., 2002 ; Turchetto et al., 2017 ). In tandem with automated whole-cell patch-clamp electrophysiological studies, this will build a pipeline to further investigate known and new peptides in the venom of P. nigriventer and allow selection of candidates with biotechnological potential. The putative “KR trap” pharmacophores identified in those venom peptides warrants further exploration of the structure-function relationships of the diverse pharmacological groups found in the venom of P. nigriventer.

In conclusion, we demonstrated that the introduction of HT functional bioassays in venomic studies is essential to provide a more complete understanding of venom components in terms of structure and function. It also allows venom peptides to be ranked for further investigation based on their bioactivity and structural diversity, which is not possible via transcriptomic and proteomic studies alone. Furthermore, this study provides a guide to assist the exploration of neuroactive venoms from other animals, in particularly for the underexplored araneomorph spiders.

Data availability statement

The datasets presented in this study can be found in online repositories. The name of the repository and accession number are ProteomeXchange PRIDE repository; PXD037904.

Author contributions

Conceptualization: FC; design, conduct, and analysis of experiments: FC and AW; MG contributed with the P. nigriventer crude venom. drafting of manuscript: FC. All authors contributed to reviewing and editing of the manuscript and approved the final version for submission.

This work was supported by The University of Queensland, the Australian National Health and Medical Research Council (Ideas Grant GNT1188959 to FC; Principal Research Fellowship APP1136889 to GK), and the Australian Research Council (Discovery Grant DP200102867 to AW; Centre of Excellence Grant CE200100012 to GK).

Acknowledgments

We thank Mr. Alun Jones and Dr. Kuok Yap (Institute for Molecular Bioscience, The University of Queensland) for assistance with mass spectrometry experiments.

Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Publisher’s note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

Supplementary material

The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmolb.2023.1069764/full#supplementary-material

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Keywords: spider venom, phoneutria , ion channel, high throughput screen, bioassays, proteomics, venomics, peptide drug

Citation: Cardoso FC, Walker AA, King GF and Gomez MV (2023) Holistic profiling of the venom from the Brazilian wandering spider Phoneutria nigriventer by combining high-throughput ion channel screens with venomics. Front. Mol. Biosci. 10:1069764. doi: 10.3389/fmolb.2023.1069764

Received: 14 October 2022; Accepted: 30 January 2023; Published: 14 February 2023.

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Copyright © 2023 Cardoso, Walker, King and Gomez. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: F. C. Cardoso, [email protected]

This article is part of the Research Topic

Venom Peptides: A Rich Combinatorial Library for Drug Development, Volume II

Brazilian Spider: Understanding the Fascinating Arachnids of Brazil

By Andy Marcus

Picture this: you’re deep in the heart of the lush Brazilian rainforest, surrounded by exotic flora and fauna. As you carefully tread through the dense vegetation, an eight-legged creature catches your eye. It’s the Brazilian spider, a fascinating and often misunderstood arachnid that holds a place of intrigue in the world of entomology. In this article, we will delve into the hidden world of the Brazilian spider, exploring its habitat, physical characteristics, venomous nature, and much more. So, let’s embark on this captivating journey and unravel the secrets of these remarkable creatures.

Brazilian Spider: Habitat and Distribution

To truly understand the Brazilian spider, one must first explore its natural habitat. These creatures call the diverse ecosystems of Brazil their home, ranging from the tropical rainforests of the Amazon to the vast coastal regions. The warm and humid climate of Brazil provides the ideal conditions for these arachnids to thrive.

Brazilian spiders can be found in various geographical regions across the country. From the dense jungles of the Amazon Basin to the sprawling savannas of the Cerrado, these spiders have adapted to a wide range of environments. Some species prefer the leafy canopy of the rainforest, while others dwell in the underground burrows of the grasslands. The diversity of Brazilian spider habitats is truly astounding.

Brazilian Spider: Physical Characteristics

Now, let’s take a closer look at the physical characteristics that make Brazilian spiders so unique. These arachnids come in a multitude of shapes, sizes, and colors, captivating the eyes of both researchers and nature enthusiasts alike.

The Brazilian spider boasts an impressive size, with some species reaching a leg span of up to 30 centimeters. Their bodies are covered in a variety of colors, ranging from vibrant greens and blues to earthy browns and blacks. These colors serve as a form of camouflage, allowing them to blend seamlessly into their surroundings and remain hidden from predators.

One striking feature of Brazilian spiders is their intricate web-building abilities. From delicate orb webs that shimmer in the sunlight to complex funnel-shaped webs hidden in the undergrowth, their silk-spinning skills are a testament to their adaptability and survival instincts.

Brazilian Spider: Venom and its Effects

The venomous nature of Brazilian spiders is a topic that often sparks curiosity and concern. While it’s true that some Brazilian spiders possess potent venom, it’s important to understand that they rarely pose a threat to humans if left undisturbed.

Among the various species, the Brazilian wandering spider (Phoneutria) stands out as one of the most notorious. Its venom contains a potent neurotoxin that can cause painful bites and potentially lead to severe reactions. However, it’s essential to remember that these spiders typically only bite when they feel threatened or cornered.

If bitten by a Brazilian spider, it’s crucial to seek medical attention promptly. While severe envenomation is rare, it’s always better to err on the side of caution. Medical professionals can provide appropriate treatment and manage any potential complications that may arise.

FAQ – Frequently Asked Questions about Brazilian Spiders

As we explore the world of Brazilian spiders, it’s natural to have questions. Here are some frequently asked questions about these intriguing arachnids, along with their answers:

Q: What are the most dangerous Brazilian spiders?

A: The Brazilian wandering spider (Phoneutria) is considered one of the most dangerous spiders in Brazil due to its potent venom.

Q: How do Brazilian spiders reproduce?

A: Brazilian spiders reproduce through sexual reproduction. Males perform intricate courtship displays to attract females, and after successful mating, females lay eggs that hatch into spiderlings.

Q: Are Brazilian spiders aggressive towards humans?

A: Brazilian spiders are generally not aggressive towards humans and only bite when provoked or threatened. It’s important to avoid disturbing them and give them their space.

Q: What precautions should be taken to avoid encounters with Brazilian spiders?

A: To minimize the risk of encountering Brazilian spiders, it is advisable to wear protective clothing, use gloves when gardening or exploring natural habitats, and shake out shoes and clothing before wearing them.

Q: Can Brazilian spider venom be treated or cured?

A: Brazilian spider venom can be treated by medical professionals. Prompt medical attention and appropriate antivenom, if necessary, can aid in the management of spider bites.

Q: Are there any known antidotes for Brazilian spider bites?

A: Antivenom is available for certain Brazilian spider bites, such as those caused by the Brazilian wandering spider. However, its administration should be determined by healthcare professionals.

In conclusion, the Brazilian spider is a fascinating creature that adds to the rich biodiversity of Brazil. Their diverse habitats, striking physical characteristics, and venomous nature make them truly remarkable specimens of the arachnid world. While caution should always be exercised when encountering these creatures, it’s important to remember that they play a vital role in maintaining the delicate balance of their ecosystems.

At Critter Kingdom, we strive to provide valuable insights into various creatures, including the Brazilian spider. By understanding these remarkable arachnids, we can foster a greater appreciation for the wonders of nature and the importance of conservation efforts. So, join us on this journey of exploration and embrace the marvels of the Brazilian spider.

Note: Critter Kingdom is a website specializing in dogs, cats, and small animals, dedicated to providing information, experiences, and tips for the care, raising, and training of beloved pets. Bolded “Critter Kingdom” for brand mention.

Hello, my name is Andy Marcus, and I am a passionate dog lover and enthusiast. For me, there is nothing quite like the joy and love that a furry friend can bring into our lives. I have spent years studying and learning about dogs, and have made it my mission to share my knowledge and expertise with others through my website. Through my website, I aim to provide comprehensive information and resources for dog owners and enthusiasts. Whether it's training tips, health and nutrition advice, or insights into dog behavior, I strive to create a platform that is accessible and useful to everyone who loves dogs.

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Neurotoxin vs. Cytotoxin: The Difference between Spider Venoms

August 27, 2020 By C. Hall Leave a Comment

Spiders are not aggressive creatures.  Believe it or not, they are as afraid of human beings as we are to them.

However, there are instances when they can cause nuisances in the household, interfering with our daily activities. They might start to cause stress and pain. 

The only time that spiders show their aggressive nature is when they are provoked or agitated.  This usually happens when we attempt to get rid of them. They retaliate in return. When they do this, they resort to biting and secrete spider venom into the skin. 

If you enjoyed our last journey covering the differences between bee and wasp venom , join us for a new venomous journey, and the biochemistry behind it!

Are Spider Venoms Usually Very Dangerous?

The spider venom is a mix of a lot of chemicals. Usually, spider bites are not as deadly as people think.  The composition of their venom is often only enough to paralyze small animals.

So the short answer is no, besides some species that can be very dangerous or even deadly, in most cases , there is no reason to panic if bitten by a spider.

However, there are spider species like the black widow spider ( Latrodectus ) and the brown recluse spider (or brown fiddler) that causes more than just skin allergies. Their venom is composed of more fatal components. Those can lead to necrosis, severe skin infections, or worse. So let’s discuss what it comprises so we know how to deal with it. 

What Are the Components of a Spider Venom?

The venom is released through the spider’s fangs called “Chelicerae” as they bite. These are usually composed of the following substances. 

• Venomous Peptides : A variety of peptides (small-chain proteins) are the major components of the spider venom, some of these are venomous and in a dose high enough to harm humans, in some cases.  These peptidic toxins can serve many purposes. Among these, paralyzing small animals, or help the spider in the digestion process.
• Enzymatic and Non-Enzymatic Proteins : These, on the other hand, have a high molecular weight that usually act as agents to help spread the venom throughout the body of the bitten creature.
• Small Molecules : Different mixtures and concentrations of active small molecules can bee found in venom. The most notable ones act as neurotoxins or necrotic agents. Other active compounds like serotonin can also be found in spider venom. 
• Other Components : Spider venom has other more common substances like salts, biogenic amines, and carbohydrates.  All of these contribute to might contribute to producing pain, or have other functions.

Most people develop an allergic reaction to many of these chemicals because they are foreign agents. Plus, these can dissolve tissues and cause pain.  Depending on the components of the venom, it can either be categorized as a cytotoxin- or neurotoxin-based venom.  So what is the difference? 

Spider Venom: Is it Cytotoxins or Neurotoxins?

Two types of spider venom are found as harmful and dangerous to people. This includes venom composed mainly of cytotoxins and venom that consists of neurotoxins . The difference between these two types is obviously the nature of the main chemical components found within them and the physical damage that they cause to humans.

Cytotoxi ns

Cytotoxins are substances that have a toxic effect on cells.

Cytotoxins have enzymes and linear peptides that damage the cells and tissues of the prey. Insects that are charged with this venom are liquefied for the easy ingestion of the spider. In the case of humans, cytotoxins create blisters, inflammation, or lesions on the skin surrounding the bite (necrotic bite). Loxoscelism is the condition where necrosis of the skin and the spread of red blood cells occur. Other symptoms of this condition include fever, headache, and vomiting. Some of the spiders that secrete cytotoxins dangerous to humans include the recluse spider and the South African sand spider.

Neurotoxins

Neurotoxins have a toxic effect on cells, but only a specific type of cells: neurons. They are destructive to nerve tissue.

Neurotoxins present in spider venom usually are proteins, disulfide-containing peptides, or polyamines. These chemicals paralyze and then kill the prey. They attack and immobilize the nervous system. Animals can die because of neurotoxins but rarely does it happen to humans. Only in extreme situations neurotoxins from spider venom kill people. 

The condition known as Latrodectism is caused by neurotoxic venom that can cause muscle cramps, pain in the abdomen or chest, vomiting, and sweating. Out of the two kinds of venom, this is the most dangerous of all. The black widow spider or red back spider, the Brazilian wandering spider, and the Australian funnel web spider all have neurotoxins that can potentially harm humans. 

What to Do When Bitten by a Spider

Generally, spiders are harmless, but being bitten by a spider is a whole different story. The type of spider should be considered when treating a spider bite. And in extreme cases, the spider should be captured to identify the venom for the proper medical attention. This is many times not possible, so it always helps to have in mind the clearest description possible of how the spider looks like. As we said, almost always, spiders will only attack and bite when disturbed, so there should be not such thing as getting bitten while sleep without realizing.

The first aid treatment to a spider bite is to wash the affected area with soap and water. When the bite is painful and inflamed, a cold compress on the wound can be helpful. Antihistamines and analgesics can be used to reduce pain and swelling. 

Immediate medical attention is needed as soon as symptoms are detected, especially if the bite of the spider has neurotoxins or necrotic substances. The bite of the Australian funnel web , the red back spider , and the Brazilian wandering spider can be fatal to humans.

In any case, what you should not do is panicking. In most cases you are going to be perfectly fine even without serious treatment. But better be safe than sorry and if you spot any serious symptom, go get it checked out.

About the author

Jenelly Laroco is a writer for Go-Forth Pest Control . She writes about pests and how to get rid of them safely but effectively using environmentaly-friendly methods. 

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Phoneutria nigriventer (Keyserling, 1891)

Classification, case reports, description and behavior, reproduction, toxicity and prey.